NCK-Associated Protein 1-Like (NCKAP1L) antibody

Details for Product No. ABIN451731
Antigen
  • HEM1
  • 4930568P13Rik
  • AI463083
  • Hem1
  • Hemp1
  • NCK associated protein 1 like
  • zgc:172352
  • nck-associated protein 1-like
  • NCK-associated protein 1-like
  • NCKAP1L
  • zgc:172352
  • LOC100030968
  • nckap1l
  • LOC100618382
  • LOC100639088
  • Nckap1l
Alternatives
anti-Human NCK-Associated Protein 1-Like antibody for ELISA
Reactivity
Dog (Canine), Horse (Equine), Human, Mouse (Murine), Rat (Rattus)
51
34
26
10
8
7
6
4
3
3
2
1
1
Host
Goat
34
18
Clonality
Polyclonal
Conjugate
Un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA
23
20
13
7
2
Options
Immunogen Peptide with sequence C-RHTENVTKTKTPE corresponding to internal region according to NP_005328.2.
Purification Immunogen affinity purified
Antigen
Alternative Name HEM1 (NCKAP1L Antibody Abstract)
Background Gene Symbol: NCKAP1L
Gene ID 3071
NCBI Accession NP_005328
Pathways Regulation of Actin Filament Polymerization
Application Notes Peptide ELISA Detection limit 1:16000WB: Preliminary experiments gave an approx. 50 kDa band in human tonsil and thymus lysates after 0.5 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 128 kDa band according to NP_005328.2. The 50 kDa band was successfully blocked by incubation with the immunizing peptide.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer 0.5 mg/mL Tris ( pH 7.3) and 0.5 % BSA
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Store at -20°C. Avoid freeze-thaw cycles.
Product cited in: Weiner, Rentel, Ott, Brown, Jedrychowski, Yaffe, Gygi, Cantley, Bourne, Kirschner: "Hem-1 complexes are essential for Rac activation, actin polymerization, and myosin regulation during neutrophil chemotaxis." in: PLoS biology, Vol. 4, Issue 2, pp. e38, 2006 (PubMed).

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