CD3 antibody

Catalog No. ABIN457415

Product Details anti-CD3 Antibody

Target: CD3

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD3 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB), Immunocytochemistry (ICC)

Specificity: The mouse monoclonal antibody APA1/1 recognizes an activation-dependent intracellular epitope of CD3 epsilon. Exposure of the epitope precedes CD3 phosphorylation and recruitment and activation of ZAP70, which initiates the signaling cascade produced by T-cell activation. APA1/1 provides the earliest known marker for TCR-mediated T cell activation.

Cross-Reactivity (Details): Human, Mouse

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Purified human CD3 proteins isolated from thymus

Clone: APA1-1

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: Intracellular staining, recommended dilution: 1-4 μg/mL, positive control: human T cells stimulated with anti-CD3 (MEM-57) antibody (1 μg/mL). Sample preparation: At the end of stimulation of T cells, perform staining of surface markers (if required) in PBS + 0.1 % BSA for 20 min. on ice. Wash with PBS and fix with 2 % formaldehyde, 30 min on ice. Wash with PBS and incubate in PBS + 0,1 % saponine, 5 min. RT. Incubate the cells in PBS + 1 % BSA + 0.03 % saponine, 15 min. on ice. Incubate with fluorescence-labeled APA1/1 antibody (1-2 μg/mL) in PBS + 1 % BSA + 0.03 % saponine in dark, 20 min. RT. Wash with PBS + 1 % BSA + 0.03 % saponine, resuspend in PBS.
Immunocytochemistry: Fixed and permeabilised cells. The antibody can distinguish TCR-stimulated from non-stimulated cells.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-CD3 antibody (ABIN457415)

Tailor, Tsai, Shameli, Serra, Wang, Robbins, Nagata, Szymczak-Workman, Vignali, Santamaria: "The proline-rich sequence of CD3epsilon as an amplifier of low-avidity TCR signaling." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 181, Issue 1, pp. 243-55, (2008) (PubMed).

Wong, Dutly, Sacher, Lee, Hwang, Liu, Keshavjee, Hu, Waddell: "Targeted cell replacement with bone marrow cells for airway epithelial regeneration." in: American journal of physiology. Lung cellular and molecular physiology, Vol. 293, Issue 3, pp. L740-52, (2007) (PubMed).

Rieux-Laucat, Hivroz, Lim, Mateo, Pellier, Selz, Fischer, Le Deist: "Inherited and somatic CD3zeta mutations in a patient with T-cell deficiency." in: The New England journal of medicine, Vol. 354, Issue 18, pp. 1913-21, (2006) (PubMed).

Gil, Schrum, Alarcón, Palmer: "T cell receptor engagement by peptide-MHC ligands induces a conformational change in the CD3 complex of thymocytes." in: The Journal of experimental medicine, Vol. 201, Issue 4, pp. 517-22, (2005) (PubMed).

Torres, Alcover, Zapata, Arnaud, Pacheco, Martín-Fernández, Villasevil, Sanal, Regueiro: "TCR dynamics in human mature T lymphocytes lacking CD3 gamma." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 170, Issue 12, pp. 5947-55, (2003) (PubMed).

Delgado, Fernández, Dave, Kappes, Alarcón: "CD3delta couples T-cell receptor signalling to ERK activation and thymocyte positive selection." in: Nature, Vol. 406, Issue 6794, pp. 426-30, (2000) (PubMed).

Alarcón, Ley, Sánchez-Madrid, Blumberg, Ju, Fresno, Terhorst: "The CD3-gamma and CD3-delta subunits of the T cell antigen receptor can be expressed within distinct functional TCR/CD3 complexes." in: The EMBO journal, Vol. 10, Issue 4, pp. 903-12, (1991) (PubMed).

Target Details for CD3

Target: CD3

Alternative Name: CD3 (CD3 Products)

Synonyms: CD3 antibody, AI504783 antibody, CD3epsilon antibody, T3e antibody, cd3-gamma antibody, cd3g antibody, cd3g-a antibody, cd3g/d antibody, cd3gd antibody, t3g antibody, CD3e molecule antibody, CD3d molecule antibody, CD3 antigen, epsilon polypeptide antibody, CD3g molecule S homeolog antibody, CD3E antibody, CD3D antibody, Cd3e antibody, cd3g.S antibody

Background: CD3 antigen, epsilon polypeptide,CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.,CD3 epsilon chain, T3E, IMD18

Gene ID: 916

UniProt: P07766

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway