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Goat anti-Cat IgA, IgG, IgM (Heavy & Light Chain) Antibody

ID, IEP, RID Polyclonal IgG unconjugated
Catalog No. ABIN457614
  • Target See all IgA, IgG, IgM products
    IgA, IgG, IgM
    Binding Specificity
    • 74
    • 58
    • 31
    • 16
    • 9
    Heavy & Light Chain
    Reactivity
    • 72
    • 47
    • 36
    • 28
    • 12
    • 7
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    Cat
    Host
    • 160
    • 55
    • 4
    • 3
    • 1
    Goat
    Clonality
    • 218
    • 1
    Polyclonal
    Application
    • 117
    • 112
    • 77
    • 73
    • 68
    • 56
    • 25
    • 24
    • 23
    • 21
    • 15
    • 15
    • 13
    • 12
    • 11
    • 8
    • 2
    • 1
    • 1
    • 1
    • 1
    Immunodiffusion (ID), Immunoelectrophoresis (IEP), Radial Immunodiffusion (RID)
    Specificity
    The reactivity of the antiserum is directed to the major isotypes of the cat immunoglobulin system as tested by immunoelectrophoresis and double radial immunodiffusion against pooled serum and purified immunoglobulinsPrecipitating polyclonal goat antiserum to cat immunoglobulins, heavy and light chains
    Characteristics
    Total protein and IgG concentration in the antiserum are comparable to those of pooled goat serum. Antibody titre: Precipitin titre not less than 1:64 when tested against normal cat serum in agar block titration.
    Purification
    Delipidated, heat inactivated, lyophilized stable whole serum
    Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibody activity with other serum protein. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.
    Immunogen
    Purified normal IgG and fractions containing IgA and IgM and isolated from cat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
    Isotype
    IgG
  • Application Notes
    In precipitating techniques as immunoelectrophoresis and single and double radial immunodiffusion (Mancini, Ouchterlony) to identify and measure immunoglobulins, as secondary antibody to precipitate the immunoglobulin in normal cat serum.
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    It is reconstituted by adding 1 mL sterile distilled water.
    Buffer
    No foreign proteins added.
    Preservative
    Without preservative
    Handling Advice
    Working dilutions are prepared by adding sterile phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 °C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
    Storage
    4 °C/-20 °C
    Storage Comment
    The lyophilized antiserum is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.
  • Target
    IgA, IgG, IgM
    Alternative Name
    IgG + IgA + IgM (IgA, IgG, IgM Products)
    Target Type
    Antibody
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