Sheep anti-Human IgA (Dimer) Antibody - Preadsorbed

Catalog No. ABIN458161

Product Details

Target: IgA

Binding Specificity: Dimer

Reactivity: Human

Host: Sheep

Clonality: Polyclonal

Application: Immunodiffusion (ID), Immunoelectrophoresis (IEP)

Specificity: The reactivity of the antiserum is restricted to the Fc part of the IgA molecule and to surface and hidden determinants of the secretory component as tested in immunoelectrophoresis and radial immunodiffusion. In immunoelectrophoresis using various antiserum concentrations against normal human milk two precipitin lines may be obtained, one representing IgA and the other the free secretory component. Against normal human serum only IgA is precipitated. No precipitation reaction is obtained with purified IgG, IgM, and IgG/Fab fragments

Cross-Reactivity (Details): The antiserum does not cross-react with any other component of the human Ig system. Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. of this antiserum has not been tested in detail.

Characteristics: Precipitating polyclonal sheep antiserum to human secretory IgA
Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal sheep serum. No foreign proteins added. Antibody titre: Precipitin titre 1:64 when tested against pooled normal human serum in agar-block immuno-diffusion titra
Delipidated, heat inactivated, lyophilized, stable whole antiserum

Purification: Preadsorption: Immunoaffinity adsorbed using insolubilized antigens

Immunogen: Highly purified secretory IgA isolated from pooled human milk. Freund's complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgA in human serum, milk or other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of human IgA from serum or exocrine secretions. Antisera to IgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms as secretory IgA. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. This does not exclude such use if proper controls are include.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C. Reconstitute the lyophilized antiserum by add ing 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4 °C, not ref rozen, and preferably used the same day.

Target Details

Target: IgA

Abstract: IgA Products

Target Type: Antibody