Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of different species frequently share antigenic determinants. of this antiserum has not been tested in detail.
Characteristics
Precipitating polyclonal swine antiserum to human transthyretin (prealbumin)
Purification
Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other human serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.
Immunogen
Transthyretin is the fastest protein in the immunoelectrophoresis pattern of human serum. It consists of four identical polypeptide chins with no carbohydrate. The molecular weight is 55,000 and the concentration in normal serum ranges from 100 to 400 µ g/ml. It can bind thyroxine and retinal and its concentration is reduced in severe liver diseases. Freund’s complete adjuvant is used in the first step of the immunization procedure.
In precipitating techniques as immunoelectrophoresis and single and double radial immuno-diffusion (Mancini, Ouchterlony) to identify the presence of transthyretin or to determine its concentration. The presence of non-precipitating antibodies has not been assayed. This does not exclude the use of the antiserum in non-precipitating antibody-binding techniques if proper controls are included.
Restrictions
For Research Use only
Format
Lyophilized
Concentration
Total protein and IgG concentrations in the antiserum are comparable to those of normal pooled swine serum. No foreign proteins added. Antibody titre: Precipitin titre not less than 1:64 when tested against normal human plasma in agar-block immunodiffusio
The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by add ing 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not ref rozen, and preferably used the same day.
Tested in immunoelectrophoresis and double radial immunodiffusion against normal human serum the precipitin line obtained shows a reaction of full identity with the purified immunogen. The product does not react with any other proteins component of human serum or plasma