Sheep anti-Mouse IgG (Chain kappa), (Chain lambda), (Fab Region), (Free & Bound) Antibody

Catalog No. ABIN458478

Product Details

Target: IgG

Binding Specificity: Chain kappa, Chain lambda, Fab Region, Free & Bound

Reactivity: Mouse

Host: Sheep

Clonality: Polyclonal

Application: Immunodiffusion (ID), Immunoelectrophoresis (IEP), Radial Immunodiffusion (RID)

Specificity: The antiserum is reacting with the Fab subunit of intact IgG, IgA and IgM and other Ig classes of both light chain types, with their Fab or F(ab')2 subunits and with free light chains of kappa and lambda type as tested in immunoelectrophoresis and double radial immunodiffusion

Characteristics: Precipitating polyclonal sheep antiserum to mouse Fab of IgG
Total protein and IgG concentration in the antiserum are comparable to those of pooled sheep serum. Antibody titre: Precipitin titre not less than 1:64 when tested against normal mouse serum in agar block titration.

Purification: Delipidated, heat inactivated lyophilized stable whole serum
Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibodies reacting with other subunits of the immunoglobulin or reacting with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of foreign protein or immune complexes in the antiserum.

Immunogen: Purified Fab of normal mouse IgG. Freund's complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: In precipitating techniques as immunoelectrophoresis and double radial immunodiffusion to identify the presence of Fab, free or bound.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is reconstituted by adding 1 mL sterile distilled water.

Buffer: No foreign proteins added.

Preservative: Without preservative

Handling Advice: Working dilutions are prepared by adding sterile phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 °C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody