Rabbit anti-Sheep IgM (Fab Region), (Fc Region) Antibody (HRP) - Preadsorbed

Catalog No. ABIN458777

Product Details

Target: IgM

Binding Specificity: Fab Region, Fc Region

Reactivity: Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Specificity: The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgM molecule.

Characteristics: Horseradish peroxidase-conjugated IgG fraction of polyclonal rabbit antiserum to sheep IgM, heavy and light chains
Peroxidase/IgG protein molar ratio (E/P) is approximately 1.7. Enzyme marker Horseradish peroxidase enriched for isoenzyme C (RZ = 3.2).

Purification: Preadsorption: Immunoaffinity adsorbed using insolubilized antigens.

Immunogen: Purified normal IgM isolated from pooled sheep serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases, where IgM and IgG antibodies can be expected. This immunoconjugate is not pre-diluted.
The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:250, in ELISA and comparable non-precipitating antibody-binding assays between 1:2,000 and 1:10,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -24 °C.

Concentration: 10 mg/mL

Buffer: Horseradish peroxidase-coupled purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).

Preservative: Without preservative

Handling Advice: Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 °C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized conjugate is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.

Target Details

Target: IgM

Abstract: IgM Products

Target Type: Antibody