VAV3 antibody (AA 567-578)

Catalog No. ABIN461931

Product Details anti-VAV3 Antibody

Target: VAV3 (Vav 3 Oncogene (VAV3))

Binding Specificity: AA 567-578

Reactivity: Human, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This VAV3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human VAV3. This antibody is expected to recognize both reported isoforms (NP_006104.4, NP_001073343.1).

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%) Opossum (92%) Elephant, Dog, Rabbit, Pig (83%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-SGEQGTLKLPEK from an internal region of human VAV3 (NP_006104.4, NP_001073343.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%), Opossum (92%), Elephant, Dog, Rabbit, Pig (83%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:128000), WB (0.3 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for VAV3

Target: VAV3 (Vav 3 Oncogene (VAV3))

Alternative Name: VAV3 (VAV3 Products)

Synonyms: RGD1565941 antibody, A530094I06Rik antibody, AA986410 antibody, Idd18.1 antibody, vav guanine nucleotide exchange factor 3 antibody, vav 3 guanine nucleotide exchange factor antibody, vav 3 oncogene antibody, VAV3 antibody, Vav3 antibody

Background: Name/Gene ID: VAV3

Synonyms: VAV3, VAV-3, Vav 3 oncogene

Gene ID: 10451

NCBI Accession: NP_006104, NP_001073343

UniProt: Q9UKW4

Pathways: Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway