WNT3 antibody (AA 315-329)

Catalog No. ABIN461935

Product Details anti-WNT3 Antibody

Target: WNT3 (Wingless-Type MMTV Integration Site Family, Member 3 (WNT3))

Binding Specificity: AA 315-329

Reactivity: Human, Mouse, Rat, Dog, Cow, Horse, Zebrafish (Danio rerio), Bat, Chicken, Monkey, Pig, Xenopus laevis

Host: Goat

Clonality: Polyclonal

Conjugate: This WNT3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human WNT3.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Bovine, Dog, Bat, Horse, Pig, Opossum, Turkey, Chicken, Xenopus, Newt, Seabass, Stickleback, Pufferfish, Zebrafish (100%) Platypus (87%) Rabbit (80%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide CGRGHNTRTEKRKEK from an internal region of human WNT3 (NP_110380.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Bovine, Dog, Bat, Horse, Pig, Opossum, Turkey, Chicken, Xenopus, Newt, Seabass, Stickleback, Pufferfish, Zebrafish (100%), Platypus (87%), Rabbit (80%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (3.75 μg/mL), Peptide-ELISA (1:2000), WB (1:2000, 1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 3.75 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for WNT3

Target: WNT3 (Wingless-Type MMTV Integration Site Family, Member 3 (WNT3))

Alternative Name: WNT3 (WNT3 Products)

Synonyms: INT4 antibody, Ci-Wnt3 antibody, WNT3 antibody, int4 antibody, Xwnt3 antibody, wnt-3 antibody, wnt3l antibody, Xwnt-3 antibody, wnt3 antibody, Int-4 antibody, Wnt-3 antibody, wnt3-A antibody, Wnt family member 3 antibody, wingless-type MMTV integration site family, member 3 antibody, proto-oncogene Wnt-3 antibody, wingless-type MMTV integration site family member 3 antibody, Wnt family member 3A antibody, wingless-type MMTV integration site family member 3 L homeolog antibody, WNT3 antibody, Wnt3 antibody, wnt3 antibody, LOC468297 antibody, WNT3A antibody, wnt3.L antibody

Background: Name/Gene ID: WNT3
Family: WNT

Synonyms: WNT3, Proto-oncogene Int-4 homolog, Proto-oncogene Wnt-3, INT4, WNT-3 proto-oncogene protein

Gene ID: 7473

NCBI Accession: NP_110380

UniProt: P56703

Pathways: WNT Signaling, Regulation of Cell Size