BNIP3 antibody (AA 112-124)

Catalog No. ABIN461995

Product Details anti-BNIP3 Antibody

Target: BNIP3 (BCL2/adenovirus E1B 19kDa Interacting Protein 3 (BNIP3))

Binding Specificity: AA 112-124

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This BNIP3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Brand: IHC-plus™

Specificity: Species cross-reactivity: Human.

Purification: Immunoaffinity purified

Immunogen: Recombinant fragment (Residues 1-163 of Human BNIP3). Epitope: The epitope recognized by the antibody resides within amino acids 112-124 of human BNIP3 molecule.

Type of Immunogen: Recombinant protein

Isotype: IgG2b

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P (10 μg/mL), IP, WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Western Blot, Immunoprecipitation and Immunocytochemistry. Western Blot: 2-4 μg/mL. Detects a band at ~30kD and ~60kD. Positive control: Whole cell lysate from 293T cells transfected with BNIP3.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 15 mM sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: 4°C while lyophilized, once reconstituted, aliquot and store at -70°C.

Target Details for BNIP3

Target: BNIP3 (BCL2/adenovirus E1B 19kDa Interacting Protein 3 (BNIP3))

Alternative Name: NIP3 / BNIP3 (BNIP3 Products)

Background: Name/Gene ID: BNIP3

Synonyms: BNIP3, NIP3

Gene ID: 664

UniProt: Q12983

Pathways: Autophagy, Brown Fat Cell Differentiation