Membrane transport protein XK (XK) (AA 22-38) antibody

Catalog No. ABIN462089

Product Details

Target: Membrane transport protein XK (XK)

Binding Specificity: AA 22-38

Reactivity: Human, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: KLH conjugated synthetic peptide comprising amino acids 22 - 38 [LSLSSTYRSGGDRMWQA] of the human McLeod syndrome-associated Kell blood group protein (XK) protein.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset (100%) Rat, Panda, Dog, Rabbit (82%).

Purification: Protein G purified

Immunogen: Synthetic peptide from human XK. Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset (100%), Rat, Panda, Dog, Rabbit (82%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: ELISA (1:1000), IHC, IHC-P (10 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details

Target: Membrane transport protein XK (XK)

Alternative Name: KX / XK (XK Products)

Background: Name/Gene ID: XK
Subfamily: KX Blood-group Antigen
Family: Transporter

Synonyms: XK, KX, Kell complex 37 kDa component, Kx antigen, MCLDS, Membrane transport protein XK, X1k, XK-related protein 1, XKR1, XRG1

Gene ID: 7504

UniProt: P51811