CD31 antibody (Platelet/endothelial Cell Adhesion Molecule)

Details for Product anti-PECAM1 Antibody No. ABIN462134
Target
Reactivity
Human
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180
90
51
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11
9
8
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4
2
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2
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2
1
1
1
1
1
Host
Mouse
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184
68
7
3
1
1
1
Clonality (Clone)
Monoclonal ()
Conjugate
This CD31 antibody is un-conjugated
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37
32
18
11
9
8
6
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6
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5
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5
5
5
5
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5
5
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5
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5
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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84
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38
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Options
Brand IHC-plus™
Immunogen Purified recombinant fragment of human CD31 expressed in E. Coli.
Clone 2F7B2
Isotype IgG1
Specificity Human
Purification Ascites
Target
Alternative Name PECAM-1 / CD31 (PECAM1 Antibody Abstract)
Background Name/Gene ID: PECAM1

Synonyms: PECAM1, CD31, CD31 antigen, EndoCAM, PECA1, PECAM-1, GPIIA
Gene ID 5175
UniProt P16284
Pathways Regulation of Actin Filament Polymerization
Application Notes Approved: ELISA (1:10000), IF (1:200 - 1:1000), IHC, IHC-P (1:200), WB (1:100 - 1:500)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 1:200.
Comment

Target Species of Antibody: Human

Restrictions For Research Use only
Format Liquid
Concentration Lot specific
Buffer Ascitic fluid containing 0.03 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Aliquot to Avoid freeze/thaw cycles.
Storage 4 °C,-20 °C
Storage Comment Short term: 4°C
Long term: -20°C.
Images
Image no. 1 for anti-Platelet/endothelial Cell Adhesion Molecule (PECAM1) antibody (ABIN462134) Human Placenta (formalin-fixed, paraffin-embedded) stained with PECAM1 antibody ABIN4...
Image no. 2 for anti-Platelet/endothelial Cell Adhesion Molecule (PECAM1) antibody (ABIN462134) Human Spleen (formalin-fixed, paraffin-embedded) stained with PECAM1 antibody ABIN462...
Image no. 3 for anti-Platelet/endothelial Cell Adhesion Molecule (PECAM1) antibody (ABIN462134) Anti-CD31 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, par...