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PRDM5 antibody (AA 36-85)

PRDM5 Reactivity: Human, Mouse, Rat, Cow, Zebrafish (Danio rerio), Guinea Pig, Horse, Rabbit, Pig, Monkey WB, IHC, IHC (p) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN6741548
  • Target See all PRDM5 Antibodies
    PRDM5 (PR Domain Containing 5 (PRDM5))
    Binding Specificity
    • 8
    • 8
    • 6
    • 6
    • 4
    • 3
    • 1
    • 1
    • 1
    AA 36-85
    Reactivity
    • 42
    • 17
    • 6
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Human, Mouse, Rat, Cow, Zebrafish (Danio rerio), Guinea Pig, Horse, Rabbit, Pig, Monkey
    Host
    • 33
    • 9
    Rabbit
    Clonality
    • 33
    • 9
    Polyclonal
    Conjugate
    • 21
    • 4
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This PRDM5 antibody is un-conjugated
    Application
    • 30
    • 22
    • 6
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Brand
    IHC-plus™
    Specificity
    Human PRDM5
    Predicted Reactivity
    Percent identity by BLAST analysis: Human, Mouse, Rat, Bovine, Rabbit, Horse, Pig, Guinea pig (100%) Dog, Chicken (92%).
    Purification
    Immunoaffinity purified
    Immunogen
    Synthetic peptide located between aa36-85 of human PRDM5 (Q9NQX1, NP_061169). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Bovine, Rabbit, Horse, Pig, Guinea pig (100%), Dog, Turkey, Zebra finch, Chicken (92%), Bat (85%).

    Type of Immunogen: Synthetic peptide
    Isotype
    IgG
    Top Product
    Discover our top product PRDM5 Primary Antibody
  • Application Notes
    Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:1562500.
    Comment

    Target Species of Antibody: Human

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Distilled water
    Concentration
    Lot specific
    Buffer
    Lyophilized from PBS with 2 % sucrose
    Handling Advice
    Avoid repeat freeze-thaw cycles.
    Storage
    4 °C,-20 °C
    Storage Comment
    Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
    Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
  • Target
    PRDM5 (PR Domain Containing 5 (PRDM5))
    Alternative Name
    PRDM5 (PRDM5 Products)
    Synonyms
    PRDM5 antibody, BCS2 antibody, PFM2 antibody, 4432417F03Rik antibody, 6530401I24Rik antibody, AI197291 antibody, E130112L17Rik antibody, PR/SET domain 5 antibody, PR domain containing 5 antibody, PRDM5 antibody, prdm5 antibody, Prdm5 antibody
    Background
    Name/Gene ID: PRDM5

    Synonyms: PRDM5, BCS2, PFM2, PR domain containing 5, PR domain-containing protein 5
    Gene ID
    11107
    NCBI Accession
    NP_061169
    UniProt
    Q9NQX1
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