Nuclear Transcription Factor, X-Box Binding 1 (NFX1) (C-Term) antibody

Details for Product No. ABIN462262, Supplier: Log in to see
Antigen
  • NFX1
  • NFX2
  • 1300017N15Rik
  • 3000003M19Rik
  • NFX.1
  • TEG-42
  • Tex42
  • nuclear transcription factor, X-box binding 1
  • nuclear transcription factor, x-box binding 1
  • NFX1
  • CpipJ_CPIJ011986
  • nfx1
  • Nfx1
Epitope
C-Term
3
2
2
2
2
2
2
1
1
Reactivity
Cow (Bovine), Dog (Canine), Human, Monkey, Mouse (Murine), Rabbit, Rat (Rattus)
41
25
22
3
3
2
2
2
2
2
Host
Rabbit
35
6
Clonality
Polyclonal
Conjugate
Un-conjugated
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
24
14
13
7
6
4
2
1
Options
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Brand IHC-plus™
Immunogen Synthetic peptide from C-Terminus of human NFX1 (Q12986, NP_002495). Percent identity by BLAST analysis: Human, Chimpanzee, Gibbon, Marmoset (100%), Gorilla, Galago, Pig, Guinea pig (92%), Rat, Dog, Bovine, Rabbit (85%), Mouse (76%).

Type of Immunogen: Synthetic peptide
Isotype IgG
Specificity Human NFX1
Predicted Reactivity Percent identity by BLAST analysis: Human (100%) Rat, Dog, Bovine, Rabbit (85%).
Purification Immunoaffinity purified
Antigen
Alternative Name NFX1 (NFX1 Antibody Abstract)
Background Name/Gene ID: NFX1

Synonyms: NFX1, NFX2
Gene ID 4799
NCBI Accession NP_002495
UniProt Q12986
Application Notes Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:312500.
Comment

Target Species of Antibody: Human

Restrictions For Research Use only
Format Lyophilized
Reconstitution Distilled water
Concentration Lot specific
Buffer Lyophilized from PBS with 2 % sucrose
Handling Advice Avoid repeat freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Nuclear Transcription Factor, X-Box Binding 1 (NFX1) (C-Term) antibody (ABIN462262) Human Kidney (formalin-fixed, paraffin-embedded) stained with NFX1 antibody ABIN46226...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Nuclear Transcription Factor, X-Box Binding 1 (NFX1) (C-Term) antibody (ABIN462262) Human Testis (formalin-fixed, paraffin-embedded) stained with NFX1 antibody ABIN46226...
Immunohistochemistry (IHC) image for anti-Nuclear Transcription Factor, X-Box Binding 1 (NFX1) (C-Term) antibody (ABIN462262) Anti-NFX1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraf...
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