HPGD antibody (AA 255-266)

Catalog No. ABIN462285

Product Details anti-HPGD Antibody

Target: HPGD (Hydroxyprostaglandin Dehydrogenase 15-(NAD) (HPGD))

Binding Specificity: AA 255-266

Reactivity: Human, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This HPGD antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human HPGD / 15-PGDH.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset (100%) Monkey, Panda, Dog, Pig (83%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-DYDTTPFQAKTQ from the C-terminus of human HPGD / 15-PGDH (NP_000851.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset (100%), Monkey, Panda, Dog, Pig (83%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:128000), WB (0.1 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for HPGD

Target: HPGD (Hydroxyprostaglandin Dehydrogenase 15-(NAD) (HPGD))

Alternative Name: 15-PGDH / HPGD (HPGD Products)

Synonyms: CBR antibody, SDR21C1 antibody, hCBR1 antibody, 15-PGDH antibody, PGDH antibody, PGDH1 antibody, PHOAR1 antibody, SDR36C1 antibody, MGC80546 antibody, MGC79454 antibody, AV026552 antibody, carbonyl reductase 1 antibody, 15-hydroxyprostaglandin dehydrogenase antibody, hydroxyprostaglandin dehydrogenase 15-(NAD) antibody, 15-hydroxyprostaglandin dehydrogenase L homeolog antibody, hydroxyprostaglandin dehydrogenase 15 (NAD) antibody, CBR1 antibody, HPGD antibody, hpgd.L antibody, hpgd antibody, pgdh antibody, Hpgd antibody, PTRG_03214 antibody, VDBG_09957 antibody

Background: Name/Gene ID: HPGD

Synonyms: HPGD, 15-PGDH, PGDH, PGDH1, PHOAR1, Prostaglandin dehydrogenase 1, SDR36C1

Gene ID: 3248

NCBI Accession: NP_000851

UniProt: P15428