Septin 2 antibody (AA 37-49)

Catalog No. ABIN462293

Product Details anti-Septin 2 Antibody

Target: Septin 2 (SEPT2)

Binding Specificity: AA 37-49

Reactivity: Human, Mouse, Rat, Rabbit

Host: Goat

Clonality: Polyclonal

Conjugate: This Septin 2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human SEPT2 / Septin 2. Reported variants represent identical protein: NP_001008492.1, NP_006146.1, NP_004395.1, NP_001008491.1.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Mouse, Rat, Rabbit (100%) Marmoset, Bovine, Dog, Horse (92%) Panda, Bat, Pig, Chicken, Platypus (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide SKQQPTQFINPET-C from the N-terminus of human SEPT2 / Septin 2 (NP_001008491.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Mouse, Rat, Rabbit (100%), Marmoset, Bovine, Dog, Horse (92%), Panda, Bat, Pig, Chicken, Platypus (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:32000, 0.05 - 0.2 μg/mL), WB (0.05 - 0.2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL. Peptide ELISA: antibody detection limit dilution 1:32000. Western Blot: Approx. 40kD band observed in Human, Mouse and Rat Testis lysates (calculated MW of 41.5kD according to NP_001008491.1). Recommended concentration: 0.05-0.2 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Septin 2

Target: Septin 2 (SEPT2)

Alternative Name: SEPT2 / Septin 2 (SEPT2 Products)

Synonyms: DIFF6 antibody, NEDD5 antibody, Pnutl3 antibody, hNedd5 antibody, AW208991 antibody, Nedd-5 antibody, Nedd5 antibody, mKIAA0158 antibody, Vesp11 antibody, nedd5 antibody, wu:fb52g01 antibody, wu:fb99a01 antibody, Septin-2A antibody, Septin-A antibody, diff6 antibody, pnutl3 antibody, sept2 antibody, septa antibody, xlsepta antibody, SEPT2 antibody, Sep-02 antibody, Septin-2B antibody, hnedd5 antibody, sept2-B antibody, Septin-2 antibody, septin 2 antibody, septin 2 L homeolog antibody, septin 2-like antibody, COG1487; VapC; putative nucleic acid-binding protein; contains PIN domain antibody, septin 2 S homeolog antibody, SEPT2 antibody, Sept2 antibody, sept2 antibody, sept2.L antibody, SEPT2L antibody, sepT2 antibody, sept2.S antibody

Background: Name/Gene ID: SEPT2

Synonyms: SEPT2, DIFF6, HNedd5, KIAA0158, NEDD5, Septin-2, Septin 2, NEDD-5, PNUTL3

Gene ID: 4735

NCBI Accession: NP_001008491

UniProt: Q15019