GABRB3 antibody (AA 400-411)

Catalog No. ABIN462296

Product Details anti-GABRB3 Antibody

Target: GABRB3 (gamma-aminobutyric Acid (GABA) A Receptor, beta 3 (GABRB3))

Binding Specificity: AA 400-411

Reactivity: Human, Rat, Mouse, Dog, Horse, Monkey, Hamster

Host: Goat

Clonality: Polyclonal

Conjugate: This GABRB3 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human GABRB3. This antibody is expected to recognize both reported isoforms (NP_000805.1, NP_068712.1).

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Cat, Dog, Horse (100%) Bovine, Rabbit, Pig, Lizard (92%) Opossum, Turkey, Platypus (83%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-EKTAKAKNDRSK from an internal region of human GABRB3 (NP_000805.1, NP_068712.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Cat, Dog, Horse (100%), Bovine, Rabbit, Pig, Lizard (92%), Opossum, Turkey, Platypus (83%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:8000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL. Peptide ELISA: antibody detection limit dilution 1:8000.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for GABRB3

Target: GABRB3 (gamma-aminobutyric Acid (GABA) A Receptor, beta 3 (GABRB3))

Alternative Name: GABRB3 (GABRB3 Products)

Synonyms: GABRB3 antibody, eca5 antibody, LOC100230475 antibody, ECA5 antibody, A230092K12Rik antibody, AW049585 antibody, Cp1 antibody, Gabrb-3 antibody, beta3 antibody, gamma-aminobutyric acid type A receptor beta3 subunit antibody, gamma-aminobutyric acid (GABA) A receptor, beta 3 antibody, gamma-aminobutyric acid type A receptor beta 3 subunit antibody, gamma-aminobutyric acid (GABA) A receptor, subunit beta 3 antibody, GABRB3 antibody, gabrb3 antibody, Gabrb3 antibody

Background: Name/Gene ID: GABRB3
Subfamily: GABA A receptor
Family: Ion Channel

Synonyms: GABRB3, Cleft palate 1, Cp1, GABAA receptor beta-3 subunit, GABA(A) receptor, beta 3, ECA5, GABA A receptor beta 3

Gene ID: 2562

NCBI Accession: NP_000805, NP_068712

UniProt: P28472

Pathways: Sensory Perception of Sound