SNAP25 antibody (Synaptosomal-Associated Protein, 25kDa)

Details for Product anti-SNAP25 Antibody No. ABIN462370
Target
Reactivity
Hamster, Human, Pig, Rat
164
104
97
33
25
12
12
11
11
8
6
5
3
2
2
2
2
2
2
1
1
1
1
1
Host
Mouse
107
68
10
4
1
Clonality (Clone)
Monoclonal ()
Conjugate
This SNAP25 antibody is un-conjugated
11
8
6
6
6
5
4
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
135
96
67
37
34
23
19
14
13
12
10
9
9
2
2
2
1
1
Options
Brand IHC-plus™
Immunogen Crude human synaptic immunoprecipitate.

Type of Immunogen: Protein extract
Clone SP12
Isotype IgG1
Specificity Recognizes the human pre-synaptic protein, SNAP-25, also known as Synaptosomal-associated protein 25, Super protein (SUP) or Synaptosomal-associated 25 kD protein. SNAP-25 is a 206 amino acid presynaptic protein of ~ containing two domains. Will recognize SNAP-25 fusion protein from COS cells, but not the fusion protein from bacterial systems. has been used to study the distribution of synaptic changes in the hippocampus of patients with medically refractory temporal lobe epilepsy.
Purification Tissue culture supernatant
Target
Alternative Name SNAP25 (SNAP25 Antibody Abstract)
Background Name/Gene ID: SNAP25
Family: SNAP-25

Synonyms: SNAP25, BA416N4.2, DJ1068F16.2, SNAP, SUP, Super protein, RIC4, SEC9, SNAP-25, RIC-4
Gene ID 6616
UniProt P60880
Pathways Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport
Application Notes Approved: ELISA, IHC, IHC-P (0.2 - 0.5 μg/mL), WB (1:500 - 1:5000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 0.2-0.5 μg/mL.
Comment

Target Species of Antibody: Human

Restrictions For Research Use only
Format Liquid
Concentration Lot specific
Buffer PBS,  pH 7.2, 0.09 % sodium azide. Sourced in TCS.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C,-20 °C
Storage Comment 4°C or -20°C, Avoid freeze-thaw cycles.
Images
Image no. 1 for anti-Synaptosomal-Associated Protein, 25kDa (SNAP25) antibody (ABIN462370) Human Brain, Cerebellum (formalin-fixed, paraffin-embedded) stained with SNAP25 antib...
Image no. 2 for anti-Synaptosomal-Associated Protein, 25kDa (SNAP25) antibody (ABIN462370) Anti-SNAP25 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin...