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eIF4EBP1 antibody (AA 101-118)

EIF4EBP1 Reactivity: Human, Mouse, Rat, Rabbit, Hamster WB, IHC, IHC (p), GS Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN462434
  • Target See all eIF4EBP1 (EIF4EBP1) Antibodies
    eIF4EBP1 (EIF4EBP1) (Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1))
    Binding Specificity
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    AA 101-118
    Reactivity
    • 236
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    • 120
    • 21
    • 14
    • 7
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    • 3
    • 2
    • 2
    • 2
    • 1
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    Human, Mouse, Rat, Rabbit, Hamster
    Host
    • 255
    • 13
    • 4
    Rabbit
    Clonality
    • 236
    • 38
    Polyclonal
    Conjugate
    • 115
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    • 1
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    • 1
    • 1
    • 1
    This eIF4EBP1 antibody is un-conjugated
    Application
    • 216
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    Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Gel Shift (GS)
    Brand
    IHC-plus™
    Specificity
    Detects multiple bands at 19-25 kD, corresponding to the apparent molecular mass of the various phosphorylation states of PHAS-I protein on SDS-PAGE immunoblots. This antibody provides a simple read-out for PKB (Akt) activity in vivo through the use of gel-shift assays. Antibody specificity has been confirmed in Western blot peptide competition studies.
    Predicted Reactivity
    Percent identity by BLAST analysis: Human, Gorilla, Mouse, Rat, Hamster, Rabbit (100%) Marmoset, Goat, Elephant, Dog, Bat, Bovine, Horse (94%) Panda (83%).
    Purification
    Immunoaffinity purified
    Immunogen
    An 18 residue synthetic peptide SPEDKRAGGEESQFEMDI based on the human PHAS-I (residues 101-118) and the peptide coupled to KLH. This sequence is identical to rat and mouse PHAS- I over these residues. Percent identity by BLAST analysis: Human, Gorilla, Mouse, Rat, Hamster, Rabbit (100%), Marmoset, Goat, Elephant, Dog, Bat, Bovine, Horse (94%), Panda (83%).

    Type of Immunogen: Synthetic peptide - KLH conjugated
    Top Product
    Discover our top product EIF4EBP1 Primary Antibody
  • Application Notes
    Approved: GS, IHC, IHC-P (5 - 10 μg/mL), WB (4 μg/mL)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.
    Comment

    Target Species of Antibody: Human

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    BBS,  pH 8.4 (25 mM sodium borate, 100 mM boric acid, 75 mM sodium chloride, 5 mM EDTA)
    Handling Advice
    avoid freeze thaw cycles. For maximum product recovery, after thawing, centrifuge the product vial before removing cap.
    Storage
    4 °C,-20 °C
    Storage Comment
    Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. For maximum product recovery, after thawing, centrifuge the product vial before removing cap.
  • Target
    eIF4EBP1 (EIF4EBP1) (Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1))
    Alternative Name
    EIF4EBP1 / 4EBP1 (EIF4EBP1 Products)
    Synonyms
    4E-BP1 antibody, 4EBP1 antibody, BP-1 antibody, PHAS-I antibody, 4e-bp1 antibody, AA959816 antibody, 153432_at antibody, 43-BP antibody, 4E-BP antibody, 4EBP antibody, 4e-bp antibody, 4eBP antibody, 4ebp antibody, BcDNA.HL08053 antibody, BcDNA:HL08053 antibody, CG8846 antibody, CT8705 antibody, Dm 4E-BP antibody, Dmel\\CG8846 antibody, Phas1 antibody, Thor/EIF4EBP antibody, anon-WO0172774.158 antibody, d4E-BP antibody, d4E-BP1 antibody, d4EBP antibody, d4e-bp antibody, d4eBP antibody, dthor antibody, eIF4E-BP antibody, l(2)06270 antibody, l(2)k07736 antibody, l(2)k13517 antibody, pp20 antibody, thor antibody, EIF4EBP1 antibody, fc04h09 antibody, wu:fc04h09 antibody, zgc:64137 antibody, eukaryotic translation initiation factor 4E binding protein 1 antibody, CG8846 gene product from transcript CG8846-RA antibody, EIF4EBP1 antibody, Eif4ebp1 antibody, Thor antibody, eif4ebp1 antibody
    Background
    Name/Gene ID: EIF4EBP1

    Synonyms: EIF4EBP1, 4E-BP1, 4EBP1, BP-1, PHAS-I, EIF4E-binding protein 1
    Gene ID
    1978
    UniProt
    Q13541
    Pathways
    MAPK Signaling, PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Regulation of Cell Size, BCR Signaling
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