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GM-CSF antibody (Biotin)

CSF2 Reactivity: Mouse WB, ELISA, Neut Host: Rabbit Polyclonal Biotin
Catalog No. ABIN464928
  • Target See all GM-CSF (CSF2) Antibodies
    GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))
    Reactivity
    • 110
    • 39
    • 20
    • 10
    • 10
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    Mouse
    Host
    • 90
    • 31
    • 29
    • 4
    • 1
    Rabbit
    Clonality
    • 96
    • 59
    Polyclonal
    Conjugate
    • 84
    • 22
    • 8
    • 7
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This GM-CSF antibody is conjugated to Biotin
    Application
    • 120
    • 69
    • 32
    • 30
    • 29
    • 25
    • 17
    • 16
    • 15
    • 11
    • 7
    • 7
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Western Blotting (WB), ELISA, Neutralization (Neut)
    Specificity
    Mouse GM-CSF
    Purification
    Immunoaffinity purified
    Immunogen
    E.coli derived recombinant Murine GM-CSF.

    Type of Immunogen: Cell extract
    Top Product
    Discover our top product CSF2 Primary Antibody
  • Application Notes
    Approved: ELISA (1 μg/mL), Neut, WB (0.1 - 0.2 μg/mL)

    Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect mGM-CSF by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant mGM-CSF. To detect mGM-CSF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Murine GM-CSF (LS-C104755) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mGM-CSF. Western Blot: To detect mGM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mGM-CSF is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
    Comment

    Target Species of Antibody: Mouse

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Sterile PBS containing 0.1 % BSA
    Concentration
    Lot specific
    Buffer
    Lyophilized from PBS,  pH 7.2
    Handling Advice
    Avoid freeze/thaw cycles.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store Lyophilized at room temperature up to 1 month
    Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
  • Target
    GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))
    Alternative Name
    CSF2 / GMCSF / GM-CSF (CSF2 Products)
    Synonyms
    GMCSF antibody, GM-CSF antibody, CSF2 antibody, Csfgm antibody, Gm-CSf antibody, MGI-IGM antibody, Gm-csf antibody, Gmcsf antibody, CSF antibody, colony stimulating factor 2 antibody, colony stimulating factor 2 (granulocyte-macrophage) antibody, CSF2 antibody, Csf2 antibody
    Background
    Name/Gene ID: CSF2

    Synonyms: CSF2, Colony-stimulating factor, CSF, GM-CSF, Sargramostim, Molgramostin, GMCSF
    Gene ID
    1437
    UniProt
    P04141
    Pathways
    JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin
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