Osteoprotegerin antibody

Catalog No. ABIN464948

Product Details anti-Osteoprotegerin Antibody

Target: Osteoprotegerin (TNFRSF11B) (Tumor Necrosis Factor Receptor Superfamily, Member 11b (TNFRSF11B))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Osteoprotegerin antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: Human OPG

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human OPG.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (0.5 - 2 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Immunohistochemistry: To detect Human OPG by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with LifeSpan's Biotinylated Anti-Human OPG (LS-C104334) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human OPG. ELISA: To detect hOPG by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human OPG (LS-C104333) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hOPG. Western Blot: To detect hOPG by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hOPG is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for Osteoprotegerin

Target: Osteoprotegerin (TNFRSF11B) (Tumor Necrosis Factor Receptor Superfamily, Member 11b (TNFRSF11B))

Alternative Name: Osteoprotegerin / OPG (TNFRSF11B Products)

Synonyms: CD254 antibody, ODF antibody, OPGL antibody, OPTB2 antibody, RANKL antibody, TRANCE antibody, hRANKL2 antibody, sOdf antibody, OCIF antibody, OPG antibody, TR1 antibody, Opg antibody, ocif antibody, opg antibody, tr1 antibody, TNF superfamily member 11 antibody, TNF receptor superfamily member 11b antibody, tumor necrosis factor receptor superfamily, member 11b (osteoprotegerin) antibody, TNF receptor superfamily member 11B antibody, tumor necrosis factor receptor superfamily member 11b S homeolog antibody, tumor necrosis factor receptor superfamily, member 11b antibody, TNFSF11 antibody, TNFRSF11B antibody, Tnfrsf11b antibody, tnfrsf11b.S antibody

Target Type: Chemical

Background: Name/Gene ID: TNFRSF11B
Family: TNF Receptor

Synonyms: TNFRSF11B, OCIF, OPG, Osteoprotegerin, TR1

Gene ID: 4982

UniProt: O00300