CXCL10 antibody
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- Target See all CXCL10 Antibodies
- CXCL10 (Chemokine (C-X-C Motif) Ligand 10 (CXCL10))
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This CXCL10 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Neutralization (Neut)
- Specificity
- Human IP-10
- Purification
- Immunoaffinity purified
- Immunogen
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E.coli derived recombinant Human IP-10.
Type of Immunogen: Cell extract - Top Product
- Discover our top product CXCL10 Primary Antibody
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- Application Notes
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Approved: ELISA (0.5 - 2 μg/mL), Neut (2 - 3 μg/mL), WB
Usage: Immunohistochemistry: This antibody stained formalin-fixed paraffin-embedded sections of human carcinoid tissue. The recommended concentration is 1 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human IP-10 (100 ng/mL), a concentration of 5-8 μg/mL of this antibody is required. ELISA: To detect hIP-10 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IP-10 (LS-C104616) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIP-10. Western Blot: To detect hIP-10 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIP-10 is 1.5-3 ng/lane, under either reducing or non-reducing conditions. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Sterile water
- Concentration
- Lot specific
- Buffer
- Lyophilized from PBS, pH 7.2
- Handling Advice
- Avoid freeze/thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
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Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
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- Target
- CXCL10 (Chemokine (C-X-C Motif) Ligand 10 (CXCL10))
- Alternative Name
- IP-10 / CXCL10 (CXCL10 Products)
- Synonyms
- CXCL10 antibody, C7 antibody, IFI10 antibody, INP10 antibody, IP-10 antibody, SCYB10 antibody, crg-2 antibody, gIP-10 antibody, mob-1 antibody, CRG-2 antibody, IP10 antibody, Ifi10 antibody, Scyb10 antibody, C-X-C motif chemokine ligand 10 antibody, C-X-C motif chemokine 10 antibody, chemokine (C-X-C motif) ligand 10 antibody, CXCL10 antibody, cxl10 antibody, Cxcl10 antibody
- Background
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Name/Gene ID: CXCL10
Subfamily: Intercrine alpha
Family: Intercrine
Synonyms: CXCL10, C-X-C motif chemokine 10, Gamma-IP10, IP-10, Gamma IP10, GIP-10, Mob-1, Small-inducible cytokine B10, Crg-2, IFI10, INP10, SCYB10 - Gene ID
- 3627
- UniProt
- P02778
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