GDF3 antibody

Catalog No. ABIN465020

Product Details anti-GDF3 Antibody

Target: GDF3 (Growth Differentiation Factor 3 (GDF3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GDF3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: Human GDF-3

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human GDF-3.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (0.5 - 2 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 0.125 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a  pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. ELISA: To detect hGDF-3 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human GDF-3 (LS-C104295) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hGDF-3. Western Blot: To detect hGDF-3 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hGDF-3 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for GDF3

Target: GDF3 (Growth Differentiation Factor 3 (GDF3))

Alternative Name: GDF3 (GDF3 Products)

Synonyms: KFS3 antibody, MCOP7 antibody, MCOPCB6 antibody, C78318 antibody, Gdf-3 antibody, Vgr-2 antibody, Vgr2 antibody, ecat9 antibody, RGD1564178 antibody, CVG1 antibody, der antibody, derriere antibody, gdf3-a antibody, vgr-2 antibody, vgr2 antibody, growth differentiation factor 3 antibody, growth differentiation factor 3 S homeolog antibody, GDF3 antibody, Gdf3 antibody, gdf3.S antibody

Background: Name/Gene ID: GDF3
Family: TGF beta

Synonyms: GDF3, KFS3, MCOPCB6, GDF-3, MCOP7

Gene ID: 9573

UniProt: Q9NR23