IL-5 antibody (Biotin)

Catalog No. ABIN465376

Product Details anti-IL-5 Antibody (Biotin)

Target: IL-5 (IL5) (Interleukin 5 (IL5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-5 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Specificity: Human IL-5

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human IL-5.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (1 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect Human IL-5 by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Human IL-5. To detect Human IL-5 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human IL-5 (LS-C104699) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human IL-5. Western Blot: To detect Human IL-5 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IL-5 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS containing 0.1 % BSA

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for IL-5

Target: IL-5 (IL5) (Interleukin 5 (IL5))

Alternative Name: IL5 (IL5 Products)

Synonyms: EDF antibody, IL-5 antibody, TRF antibody, Il-5 antibody, IL5 antibody, interleukin 5 antibody, IL5 antibody, Il5 antibody

Background: Name/Gene ID: IL5
Family: Hormone

Synonyms: IL5, EDF, Interleukin-5, IL-5, T-cell replacing factor, TRF

Gene ID: 3567

UniProt: P05113

Pathways: JAK-STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Feeding Behaviour