TNFSF14 antibody (Biotin)

Catalog No. ABIN465442

Product Details anti-TNFSF14 Antibody (Biotin)

Target: TNFSF14 (Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TNFSF14 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Specificity: Human LIGHT

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human LIGHT.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (1 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect hLIGHT by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hLIGHT. To detect hLIGHT by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human LIGHT (LS-C104596) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hLIGHT. Western Blot: To detect hLIGHT by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hLIGHT is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS containing 0.1 % BSA

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for TNFSF14

Target: TNFSF14 (Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14))

Alternative Name: TNFSF14 / LIGHT (TNFSF14 Products)

Synonyms: TNFSF14 antibody, CD258 antibody, HVEML antibody, LIGHT antibody, LTg antibody, TR2 antibody, HVEM-L antibody, Ly113 antibody, TNF superfamily member 14 antibody, tumor necrosis factor (ligand) superfamily, member 14 antibody, TNFSF14 antibody, Tnfsf14 antibody

Background: Name/Gene ID: TNFSF14
Family: TNF

Synonyms: TNFSF14, CD258 antigen, CD258, Delta transmembrane LIGHT, Herpesvirus entry mediator A, HVEM-L, TR2, HVEML, LIGHT, LTg

Gene ID: 8740

UniProt: O43557

Pathways: Cancer Immune Checkpoints