IL1A antibody

Catalog No. ABIN465571

Product Details anti-IL1A Antibody

Target: IL1A (Interleukin 1 alpha (IL1A))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL1A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Specificity: Mouse IL-1alpha

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Murine IL-1alpha.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (0.5 - 2 μg/mL), Neut (0.1 μg/mL), WB

Usage: Immunohistochemistry: This antibody stained colchicine injected mouse brain (including the hippocampal fissure) tissue. The primary antibody was incubated at 1 mg/mL overnight at 4 °C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA) reagent. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of mIL-1alpha (0.05 ng/mL), a concentration of 0 3-0.05 μg/mL of this antibody is required. ELISA: To detect mIL-1alpha by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine IL-1alpha (LS-C104815) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mIL-1alpha. Western Blot: To detect mIL-1alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-1alpha is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for IL1A

Target: IL1A (Interleukin 1 alpha (IL1A))

Alternative Name: IL1A (IL1A Products)

Synonyms: IL-1A antibody, IL1 antibody, IL1-ALPHA antibody, IL1F1 antibody, IL-6 antibody, Il-1a antibody, IL-1 alpha antibody, IL-1alpha antibody, L1A antibody, interleukin 1 alpha antibody, interleukin 6 antibody, IL1A antibody, IL6 antibody, Il1a antibody

Background: Name/Gene ID: IL1A
Family: IL-1

Synonyms: IL1A, Hematopoietin-1, IL-1 alpha, IL1, IL1-ALPHA, IL1 Alpha, Interleukin-1 alpha, IL-1A, IL1F1, Pro-interleukin-1-alpha, Preinterleukin 1 alpha, Interleukin 1, alpha

Gene ID: 3552

Pathways: NF-kappaB Signaling, Autophagy, Cancer Immune Checkpoints