Nerve Growth Factor antibody

Catalog No. ABIN465644

Product Details anti-Nerve Growth Factor Antibody

Target: Nerve Growth Factor (NGF)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nerve Growth Factor antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Specificity: Human beta-NGF

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human beta-NGF.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (0.5 - 2 μg/mL), Neut (5 - 8 μg/mL), WB

Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human pancreas adenocarcinoma. The recommended concentration is 0.5 mg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Optimal results were achieved without antigen retrieval. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human beta-NGF (1 ng/mL), a concentration of 0.05-0.08 μg/mL of this antibody is required. ELISA: To detect Human beta-NGF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human beta-NGF (LS-C104735) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human beta-NGF. Western Blot: To detect Human beta-NGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human beta-NGF is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for Nerve Growth Factor

Target: Nerve Growth Factor (NGF)

Alternative Name: NGF (NGF Products)

Synonyms: Beta-NGF antibody, HSAN5 antibody, NGFB antibody, Ngfb antibody, ngfb antibody, ns:zft386 antibody, xx:zft386 antibody, zgc:109730 antibody, beta-NGF antibody, hsan5 antibody, beta-ngf antibody, NGF antibody, ngf antibody, nerve growth factor antibody, nerve growth factor b (beta polypeptide) antibody, nerve growth factor S homeolog antibody, NGF antibody, Ngf antibody, ngfb antibody, ngf antibody, ngf.S antibody

Background: Name/Gene ID: NGF
Family: NGF Beta

Synonyms: NGF, Beta-nerve growth factor, ProNGF, Beta-NGF, HSAN5, NGFB

Gene ID: 4803

UniProt: P01138

Pathways: Regulation of Cell Size