IL-2 antibody

Catalog No. ABIN465672

Product Details anti-IL-2 Antibody

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Specificity: Rat IL-2

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Rat IL-2.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (0.5 - 2 μg/mL), Neut (0.1 μg/mL), WB

Usage: Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Rat IL-2 (1 ng/mL), a concentration of < 0.1 μg/mL of this antibody is required. ELISA: To detect Rat IL-2 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Rat IL-2 (LS-C104555) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat IL-2. Western Blot: To detect Rat IL-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IL-2 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for IL-2

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Alternative Name: IL2 (IL2 Products)

Synonyms: IL2 antibody, IL-2 antibody, il2 antibody, TCGF antibody, lymphokine antibody, Il-2 antibody, POIL2 antibody, interleukin 2 antibody, interleukin-15 antibody, IL2 antibody, il2 antibody, LOC100303688 antibody, Il2 antibody

Background: Name/Gene ID: IL2
Family: Interleukin

Synonyms: IL2, Aldesleukin, Interleukin 2, Lymphokine, IL-2, Interleukin-2, TCGF, T cell growth factor, T-cell growth factor

Gene ID: 3558

UniProt: P60568

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation