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IL-1 beta antibody

IL1B Reactivity: Rat WB, ELISA, Neut Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN465799
  • Target See all IL-1 beta (IL1B) Antibodies
    IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))
    Reactivity
    • 123
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    Rat
    Host
    • 175
    • 64
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    Rabbit
    Clonality
    • 181
    • 75
    • 1
    Polyclonal
    Conjugate
    • 136
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    • 2
    This IL-1 beta antibody is un-conjugated
    Application
    • 184
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    Western Blotting (WB), ELISA, Neutralization (Neut)
    Specificity
    Rat IL-1beta
    Purification
    Immunoaffinity purified
    Immunogen
    E.coli derived recombinant Rat IL-1beta.

    Type of Immunogen: Cell extract
    Top Product
    Discover our top product IL1B Primary Antibody
  • Application Notes
    Approved: ELISA (0.5 - 2 μg/mL), Neut (0.09 - 0.14 μg/mL), WB

    Usage: Immunohistochemistry: This antibody stained colchicine injected rat brain (including the ventricles and the CA3 region of the hippocampus) tissue. The primary antibody was incubated at 0.25 μg/mL overnight at 4 °C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA) reagent. Optimal concentrations and conditions may vary. Information and photo are courtesy of the Tissue Profiling group, SciLifeLab Stockholm. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Rat IL-1beta (0.20 ng/mL), a concentration of 0.09-0.14 μg/mL of this antibody is required. ELISA: To detect Rat IL-1beta by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Rat IL-1beta (LS-C104780) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat IL-1beta. Western Blot: To detect Rat IL-1beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Rat IL-1beta is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
    Comment

    Target Species of Antibody: Rat

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Sterile water
    Concentration
    Lot specific
    Buffer
    Lyophilized from PBS,  pH 7.2
    Handling Advice
    Avoid freeze/thaw cycles.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store Lyophilized at room temperature up to 1 month
    Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
  • Target
    IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))
    Alternative Name
    IL1B (IL1B Products)
    Synonyms
    IL-1 antibody, IL1-BETA antibody, IL1F2 antibody, IL-1BETA antibody, IL1beta antibody, il1-b antibody, zgc:111873 antibody, IL-1B antibody, IL-1beta antibody, Il-1b antibody, IL1B antibody, IL-1 beta antibody, IL-1b antibody, interleukin 1 beta antibody, interleukin 1, beta antibody, IL1B antibody, il1b antibody, Il1b antibody
    Background
    Name/Gene ID: IL1B
    Family: IL-1

    Synonyms: IL1B, IL-1B, IL-1 beta, IL1-BETA, Preinterleukin 1 beta, Pro-interleukin-1-beta, Catabolin, IL-1, IL1F2, Interleukin 1, beta, Interleukin-1 beta
    Gene ID
    3553
    UniProt
    P01584
    Pathways
    NF-kappaB Signaling, Interferon-gamma Pathway, TLR Signaling, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy, Cancer Immune Checkpoints, Inflammasome
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