ICAM1 antibody (Biotin)
-
- Target See all ICAM1 Antibodies
- ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))
-
Reactivity
- Human
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- This ICAM1 antibody is conjugated to Biotin
-
Application
- Western Blotting (WB), ELISA
- Specificity
- Human ICAM-1
- Purification
- Immunoaffinity purified
- Immunogen
-
E.coli derived recombinant Human ICAM-1.
Type of Immunogen: Cell extract - Top Product
- Discover our top product ICAM1 Primary Antibody
-
-
- Application Notes
-
Approved: ELISA (1 μg/mL), WB (0.1 - 0.2 μg/mL)
Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect Human ICAM-1 by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Human ICAM-1. To detect Human ICAM-1 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human ICAM-1 (LS-C104711) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human ICAM-1. Western Blot: To detect Human ICAM-1 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human ICAM-1 is 1.5-3 ng/lane, under either reducing or non-reducing conditions. - Comment
-
Target Species of Antibody: Human
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- Sterile PBS containing 0.1 % BSA
- Concentration
- Lot specific
- Buffer
- Lyophilized from PBS, pH 7.2
- Handling Advice
- Avoid freeze/thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
-
Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
-
- Target
- ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))
- Alternative Name
- ICAM1 / CD54 (ICAM1 Products)
- Synonyms
- BB2 antibody, CD54 antibody, P3.58 antibody, Icam-1 antibody, Ly-47 antibody, MALA-2 antibody, ICAM antibody, ICAM-1 antibody, ICAM1 antibody, intercellular adhesion molecule 1 antibody, intercellular adhesion molecule-1 antibody, ICAM1 antibody, Icam1 antibody, ICAM-1 antibody
- Target Type
- Viral Protein
- Background
-
Name/Gene ID: ICAM1
Family: ICAM
Synonyms: ICAM1, BB2, CD54 antigen, CD54, ICAM-1, p3.58 - Gene ID
- 3383
- UniProt
- P05362
- Pathways
- Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Regulation of Leukocyte Mediated Immunity, Thromboxane A2 Receptor Signaling
-