B-Cells antibody

Catalog No. ABIN481701

The Mouse Monoclonal anti-B-Cells antibody (Clone Ki-B1R) (ABIN481701) specifically detects B-Cells in IHC and IHC (p). The antibody is reactive with Rat samples.

Quick Overview for B-Cells antibody (ABIN481701)

Target: B-Cells

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: Ki-B1R

Product Details

Specificity: Ki-B1R recognizes a surface antigen of 47  kDa molecular weight, which appears as a single band under reducing conditions in SDS-PAGE. The epitope has not been further characterized. Antigen distribution: Isolated cells: In a test comparing the immunophenotype of rat blood lymphocytes, Ki-B1R recognised 19 % + 3 % of peripheral blood lymphocytes (n=5). In the same test, 18 % +/- 3 % of the lymphocytes stained positive for sIg, whereas 79 % +/- 3 % stained positive with Ki-T1R, the rat pan T-cell marker. Tissue sections: Ki-B1R shows a staining pattern similar to that of anti-rat immunoglobulin markers. Follicular mantle B- lymphocytes are strongly positive whereas staining of germinal centre B-lymphocytes depends on the immunological status of the lyphoid organ. In the gastrointestinal tract, the typical mucosa-associated lymphoid tissue (MALT) selectively displays the B-follicles, mostly those containing prominent germinal centres. The reactivity pattern of Ki-B1R does not change when applied in vivo. Specificity: Rat: mature B-lymphocytes, plasma cells. Other species: not tested.

Purification: Affinity purified

Immunogen: Lymphocytes

Application Details

Application Notes: Approved: IHC, IHC-P (1:100)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.5 mL distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS  pH 7.2, 5 mg/mL BSA, 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze/thaw cycles. Store undiluted.

Storage: 4 °C,-20 °C

Storage Comment: Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze/thaw cycles. Store undiluted.

Target Details

Target: B-Cells

Alternative Name: B Cell