Mouse anti-Human Ig (Chain lambda), (Light Chain) Antibody
-
- Target See all Ig products
- Ig
-
Binding Specificity
- Chain lambda, Light Chain
-
Reactivity
- Human
-
Host
- Mouse
-
Clonality
- Monoclonal
-
Application
- Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Cross-Reactivity (Details)
- Species reactivity (tested):Human. Hamster, Sheep, Goat, Rabbit, Guinea pig.
- Purification
- Protein A affinity chromatography
- Purity
- > 95 % by SDS-PAGE
- Clone
- 4C2
- Isotype
- IgG1
-
-
- Application Notes
-
Flow cytometry. Positive control: RAMOS human cell line. Immunohistochemistry on frozen and paraffin sections. ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
-
- Concentration
- 1.0 mg/mL
- Buffer
- Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C/-20 °C
- Storage Comment
-
Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer. Avoid repeatedfreezing and thawing.
Shelf life: one year from despatch. - Expiry Date
- 12 months
-
- Target
- Ig
- Alternative Name
- Immunoglobulin (Ig Products)
- Background
- Immunoglobulin classes share the same basic four polypeptide chain structure of two heavy chains (five heavy chains types) and two light chains (kappa, lambda, both having a molecular weight of 22.5 kDa). Kappa and lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of kappa to lambda is 70:30.
-