DIXDC1 antibody (Internal Region)

Catalog No. ABIN487984

Product Details anti-DIXDC1 Antibody

Target: DIXDC1 (DIX Domain Containing 1 (DIXDC1))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat, Cow, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This DIXDC1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: This antibody reacts to CCAR1.

Cross-Reactivity (Details): Species reactivity (expected):Canine, Bovine, Porcine.
Species reactivity (tested):Mouse, Rat.

Purification: Affinity chromatography

Immunogen: Peptide with sequence RYCKERPSKDKE, from the internal region (near C-Terminus) of the protein sequence according to NP_060707.2.

Application Details

Application Notes: Peptide ELISA: 1/16000. Western blot: Approx 120 kDa band observed in Mouse and Rat Skeletal Muscle lysates(calculated MW of 130 kDa according to Mouse NP_080477.1 and Rat NP_001102005.1). Recommended concentration: 1 - 3 μg/mL. An additional band of unknown identity was also consistently observed at 30 kDa. Thisband was successfully blocked by incubation with the immunizing peptide.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for DIXDC1

Target: DIXDC1 (DIX Domain Containing 1 (DIXDC1))

Alternative Name: Dixin (DIXDC1 Products)

Synonyms: CCD1 antibody, 4930563F16Rik antibody, BC048182 antibody, Ccd1 antibody, Ccd1Aalpha1L antibody, Ccd1Abeta1L antibody, Ccd1BalphaL antibody, Ccd1BbetaL antibody, Ccd1CL antibody, mKIAA1735 antibody, ccd1 antibody, dixdc1 antibody, im:7138279 antibody, DIX domain containing 1 antibody, DIX domain containing 1a antibody, DIX domain containing 1b antibody, DIXDC1 antibody, dixdc1 antibody, Dixdc1 antibody, dixdc1a antibody, dixdc1b antibody

Background: DIXDC1 belongs to the DIXDC1 family and contains 1 CH (calponin homology) domain and 1 DIX domain. The coiled coil domain mediates interaction with MAP3K4 and inhibition of AXIN1 mediated JNK activation through MAP3K4. The DIX domain mediates interaction with AXIN1 and inhibition of AXIN1 mediated JNK activation through MAP3K1. Probably also mediates interaction with DVL2.Synonyms: CCD1, Coiled-coil protein DIX1, DIX domain-containing protein 1, DIX1, DIXDC1, KIAA1735

Gene ID: 85458

NCBI Accession: NP_001033043

UniProt: Q155Q3