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Aconitase 1 antibody (C-Term, N-Term)

ACO1 Reactivity: Arabidopsis thaliana, Tomato WB Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN488534
  • Target See all Aconitase 1 (ACO1) Antibodies
    Aconitase 1 (ACO1)
    Binding Specificity
    • 20
    • 18
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term, N-Term
    Reactivity
    • 101
    • 45
    • 40
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Arabidopsis thaliana, Tomato
    Host
    • 104
    • 3
    • 1
    Rabbit
    Clonality
    • 106
    • 2
    Polyclonal
    Conjugate
    • 50
    • 12
    • 8
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 1
    This Aconitase 1 antibody is un-conjugated
    Application
    • 94
    • 39
    • 34
    • 27
    • 26
    • 20
    • 11
    • 11
    • 9
    • 4
    • 3
    • 2
    Western Blotting (WB)
    Cross-Reactivity (Details)
    Not reactive in: Chlamydomonas reinhardii
    Predicted Reactivity
    dicots including:Cucurbita maxima, Nicotiana tabacum, Ricinus communis, Solanum tuberosum, Vitis vinifera,monocots: Oryza sativa, Zea mays, trees: Picea sitchensis, Populus trichocarpa
    Characteristics
    Expected / apparent Molecular Weight of the Antigene: 98 kDa. Note that ACO1, ACO2 and ACO3 cannot be distinguished in size by standard SDS-PAGE.
    Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA, immunoblotting and enzyme inhibition.
    Purification
    serum
    Immunogen
    Arabidopsis ACO1 (AT4G35830, Q42560), codon 120 - 898 (C-terminus), was cloned in fusion with a N-terminal 6xHis tag, and over-expressed in E. coli. All recombinant protein accumulated in inclusion bodies, which were purified by centrifugation and solubilised in 6 M guanidine-HCl. The protein was refolded by dilution in 100 mM Tris-HCl 8.5, 10% (v/v) glycerol, 2 mM dithiothreitol, and concentrated prior to immunisation.
    Top Product
    Discover our top product ACO1 Primary Antibody
  • Application Notes
    1:5 000 - 1:10 000 with standard ECL (WB). At higher concentrations the antibody binds aspecifically resulting in non-specific signals âper thousand 60 kDa, including Rubisco subunits.
    Comment

    The antibody recognises all three Arabidopsis aconitase isoforms (ACO1, ACO2 and ACO3, see Bernard et al 2009). Possible differences in affinity have not been precisely quantified. Sensitivity threshold is between 2 and 10 ng for WB / ECL (see figure). Antibodies will recognize aconitase isoforms in denaturing and native gel electrophoresis.

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    For reconstitution add 100 μL of sterile water.
    Handling Advice
    Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
    Storage
    -20 °C
    Storage Comment
    store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
  • Rurek, Woyda-Ploszczyca, Jarmuszkiewicz et al.: "Biogenesis of mitochondria in cauliflower (Brassica oleracea var. botrytis) curds subjected to temperature stress and recovery involves regulation of the complexome, respiratory chain activity, ..." in: Biochimica et biophysica acta, Vol. 1847, Issue 4-5, pp. 399-417, (2015) (PubMed).

  • Target
    Aconitase 1 (ACO1)
    Alternative Name
    ACO1 (ACO1 Products)
    Synonyms
    ACONS antibody, IREB1 antibody, IREBP antibody, IREBP1 antibody, IRP1 antibody, Acon1 antibody, Ratireb antibody, ratireb antibody, Aco1 antibody, AI256519 antibody, Aco-1 antibody, Irebp antibody, Irp1 antibody, aconitase 1 antibody, aconitase 1, soluble antibody, aconitase 1 L homeolog antibody, ACO1 antibody, Aco1 antibody, aco1.L antibody
    Background
    AGI Code: At4g35830
    Aconitase is a single subunit enzyme of the tricarboxylic acid cycle (or Krebs cycle) in the mitochondria. A cytosolic isoform is also part of the glyoxylate cycle. Aconitase catalyzes the dehydration / hydration of citrate to iso-citrate, via cis-aconitate as an intermediate. The reaction is facilitated by an iron-sulphur cluster in the active site of the enzyme. The iron-sulphur cluster is somewhat unstable, especially under oxidative stress, and loss of the cofactor leads to degradation of the protein.Alternative names: ACO, citrate hydro-lyase 1,2,3
    Molecular Weight
    98 kDa. Note that ACO1 kDa, ACO2 and ACO3 cannot be distinguished in size by standard SDS-PAGE.
    UniProt
    Q42560
    Pathways
    Transition Metal Ion Homeostasis
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