PPI antibody

Catalog No. ABIN488551

This Rabbit Polyclonal antibody specifically detects PPI in IHC, ELISA, WB and IF. It exhibits reactivity toward Papaya.

Quick Overview for PPI antibody (ABIN488551)

Target: PPI

Reactivity: Papaya

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Western Blotting (WB), Immunofluorescence (IF)

Product Details

Purpose: Anti-PPI | Papain (affinity purified)

Cross-Reactivity (Details): Carica papaya
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Predicted Reactivity: Carica papaya

Purification: Immunogen affinity purified

Immunogen: Native papain isolated and purified from Carica papaya

Isotype: IgG

Application Details

Application Notes: 1 : 1000-1 : 100 000 (ELISA), (IF), (IHC), (WB)

Comment:

Antibodies have been purified using solid phase affinity chromatography and are stabilized with dextran.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 0.5 mL of sterile destilled water

Buffer: Immunogen affinity purified IgG in PBS.

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Target Details

Target: PPI

Background:

Background: Papain (EC=3.4.22.2) is a cysteine protease which belongs to peptidase C1 family. Can cause an allergic reaction in humans. Alternative names: Papaya proteinase I, allergen= Car p 1.

Additional information: Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA, immunoblotting and enzyme inhibition

Molecular Weight: 38.9 kDa