Osteocalcin antibody (AA 50-99)

Catalog No. ABIN4886487

Product Details anti-Osteocalcin Antibody

Target: Osteocalcin (BGLAP)

Binding Specificity: AA 50-99

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Osteocalcin antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Osteocalcin(BGLAP) detection. Tested with IHC-P in Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Osteocalcin(BGLAP) detection. Tested with IHC-P in Rat.
Gene Name: bone gamma-carboxyglutamate (gla) protein
Protein Name: Osteocalcin

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived rat Osteocalcin recombinant protein (Position: Y50-V99). Rat Osteocalcin shares 80.9% and 68.9% amino acid (aa) sequence identity with human and mouse Osteocalcin, respectively.

Isotype: IgG

Application Details

Application Notes: IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-Osteocalcin antibody (ABIN4886487)

Song, Wang, Zeng, Wang: "Synergistic effects of fibroblast growth factor-2 and bone morphogenetic protein-2 on bone induction." in: Molecular medicine reports, Vol. 16, Issue 4, pp. 4483-4492, (2018) (PubMed).

Liu, Ma, Wang, Zhu, Yang, Mei: "Demineralized bone matrix used for direct pulp capping in rats." in: PLoS ONE, Vol. 12, Issue 3, pp. e0172693, (2017) (PubMed).

Yang, Baban, Isales, Shi: "Role of glucocorticoid-induced leucine zipper (GILZ) in inflammatory bone loss." in: PLoS ONE, Vol. 12, Issue 8, pp. e0181133, (2017) (PubMed).

Che, Guo, Li, Wang, Wei: "Intramuscular injection of bone marrow mononuclear cells contributes to bone repair following midpalatal expansion in rats." in: Molecular medicine reports, Vol. 13, Issue 1, pp. 681-8, (2016) (PubMed).

Yin, Cheng, Qin, Yu, Yu, Zhong, Sun, Zhang: "Effects of Naringin on Proliferation and Osteogenic Differentiation of Human Periodontal Ligament Stem Cells In Vitro and In Vivo." in: Stem cells international, Vol. 2015, pp. 758706, (2015) (PubMed).

Cheng, Wang, Zhang, Shi: "The osteogenic potential of mesoporous bioglasses/silk and non-mesoporous bioglasses/silk scaffolds in ovariectomized rats: in vitro and in vivo evaluation." in: PLoS ONE, Vol. 8, Issue 11, pp. e81014, (2014) (PubMed).

Chen, Bai, Liao, Peng, Wu, Wang, Zeng, Xie: "Electrospun poly(L-lactide)/poly(?-caprolactone) blend nanofibrous scaffold: characterization and biocompatibility with human adipose-derived stem cells." in: PLoS ONE, Vol. 8, Issue 8, pp. e71265, (2013) (PubMed).

Target Details for Osteocalcin

Target: Osteocalcin (BGLAP)

Alternative Name: BGLAP (BGLAP Products)

Synonyms: BGP antibody, OC antibody, OCN antibody, Bglap1 antibody, Bglap2 antibody, OG1 antibody, mOC-A antibody, Bgp antibody, Bgpr antibody, Bgpra antibody, bgp antibody, LOC792433 antibody, ocn antibody, GLA antibody, BGLAP antibody, PMF1 antibody, bone gamma-carboxyglutamate protein antibody, bone gamma carboxyglutamate protein antibody, bone gamma-carboxyglutamate protein S homeolog antibody, bone gamma-carboxyglutamate (gla) protein antibody, osteocalcin antibody, polyamine-modulated factor 1 antibody, BGLAP antibody, Bglap antibody, bglap.S antibody, bglap antibody, ocn antibody, LOC100529177 antibody, LOC100146589 antibody

Background: Osteocalcin, also known as bone gamma-carboxyglutamic acid-containing protein (BGLAP), is a noncollagenous protein found in bone and dentin. In humans, the osteocalcin is encoded by the BGLAP gene. Its receptor is GPRC6A. It is mapped to 1q22. Osteocalcin may play a role in the body's metabolic regulation and is pro-osteoblastic, or bone-building. It acts as a hormone in the body, causing beta cellsin the pancreas to release more insulin, and at the same time directing fat cells to release the hormone adiponectin, which increases sensitivity to insulin. Also, it may play a role in male fertility. And it is found that picomolar amounts of osteocalcin affected insulin secretion and beta-cell proliferation.

Synonyms: BGLAP | Bone Gla protein | ocn | OSTCN HUMAN | Osteocalcin | P04640

Gene ID: 25295

UniProt: P04640