TLR4 antibody (N-Term)

Catalog No. ABIN4886746

Product Details anti-TLR4 Antibody

Target: TLR4 (Toll-Like Receptor 4 (TLR4))

Binding Specificity: AA 50-68, N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TLR4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Toll-like receptor 4(TLR4) detection. Tested with WB, IHC-P in Human.

Sequence: DNLPFSTKNL DLSFNPLRH

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Toll-like receptor 4(TLR4) detection. Tested with WB, IHC-P in Human.
Gene Name: toll-like receptor 4
Protein Name: Toll-like receptor 4

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human TLR4(50-68aa DNLPFSTKNLDLSFNPLRH).

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Sodium azide, Thimerosal (Merthiolate)

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-TLR4 antibody (ABIN4886746)

Sun, Li, Ma, Feng, Song, Lv, He: "Inhibition of HMGB1 reduces rat spinal cord astrocytic swelling and AQP4 expression after oxygen-glucose deprivation and reoxygenation via TLR4 and NF-κB signaling in an IL-6-dependent manner." in: Journal of neuroinflammation, Vol. 14, Issue 1, pp. 231, (2018) (PubMed).

Zhang, Fu, Tang: "The protective effects of magnolol on acute trinitrobenzene sulfonic acid‑induced colitis in rats." in: Molecular medicine reports, Vol. 17, Issue 3, pp. 3455-3464, (2018) (PubMed).

Sun, Xiu, Wang, Brigstock, Li, Qu, Gao: "Lipopolysaccharide enhances TGF-β1 signalling pathway and rat pancreatic fibrosis." in: Journal of cellular and molecular medicine, Vol. 22, Issue 4, pp. 2346-2356, (2018) (PubMed).

Sun, Xue, Xue, Ren, Wu, Wang: "Acetylpuerarin protects against OGD-induced cell injury in BV2 microglia by inhibiting HMGB1 release." in: Die Pharmazie, Vol. 73, Issue 2, pp. 92-97, (2018) (PubMed).

Gong, Guan, Tian, Li, Zhang: "Rho Kinase Type 1 (ROCK1) Promotes Lipopolysaccharide-induced Inflammation in Corneal Epithelial Cells by Activating Toll-Like Receptor 4 (TLR4)-Mediated Signaling." in: Medical science monitor : international medical journal of experimental and clinical research, Vol. 24, pp. 3514-3523, (2018) (PubMed).

Jin, Zhang, Zhao, Xu, Shao, Huang, Shi, Yang: "Responses of Intestinal Mucosal Barrier Functions of Rats to Simulated Weightlessness." in: Frontiers in physiology, Vol. 9, pp. 729, (2018) (PubMed).

Liu, Han, Chen, Huang, Zhao, Berta, Gao, Ji: "Toll-like receptor 4 contributes to chronic itch, alloknesis, and spinal astrocyte activation in male mice." in: Pain, Vol. 157, Issue 4, pp. 806-17, (2017) (PubMed).

Xu, Xue, Zhang, Qu: "Knockdown of versican V1 induces a severe inflammatory response in LPS-induced acute lung injury via the TLR2-NF-κB signaling pathway in C57BL/6J mice." in: Molecular medicine reports, Vol. 13, Issue 6, pp. 5005-12, (2017) (PubMed).

Zhou, He, Wang, Xie, Liu, Liu, Song, Ma: "Intravenous Administration Is an Effective and Safe Route for Cancer Gene Therapy Using the Bifidobacterium-Mediated Recombinant HSV-1 Thymidine Kinase and Ganciclovir." in: International journal of molecular sciences, Vol. 17, Issue 6, (2017) (PubMed).

Shanshan, Beibei, Li, Minna, Shipeng, Li, Yong: "Phospholipase A2 of Peroxiredoxin 6 Plays a Critical Role in Cerebral Ischemia/Reperfusion Inflammatory Injury." in: Frontiers in cellular neuroscience, Vol. 11, pp. 99, (2017) (PubMed).

Wang, Jiao, Zhao, Wang, Sun, Lin: "Allicin Alleviates Reticuloendotheliosis Virus-Induced Immunosuppression via ERK/Mitogen-Activated Protein Kinase Pathway in Specific Pathogen-Free Chickens." in: Frontiers in immunology, Vol. 8, pp. 1856, (2017) (PubMed).

Ansari, Li, Sun, Huang, Zhao, Cui, Hu, Zhong, Karrow, Liu: "Lipopolysaccharide induces acute bursal atrophy in broiler chicks by activating TLR4-MAPK-NF-κB/AP-1 signaling." in: Oncotarget, Vol. 8, Issue 65, pp. 108375-108391, (2017) (PubMed).

Huang, Xiao, Lu, Yang, Ansari, Khaliq, Song, Zhong, Liu, Peng: "Increased Thymic Cell Turnover under Boron Stress May Bypass TLR3/4 Pathway in African Ostrich." in: PLoS ONE, Vol. 10, Issue 6, pp. e0129596, (2016) (PubMed).

Cui, Feng, Zhang, Jia: "The anti-inflammation effect of baicalin on experimental colitis through inhibiting TLR4/NF-?B pathway activation." in: International immunopharmacology, Vol. 23, Issue 1, pp. 294-303, (2015) (PubMed).

Xu, Wang, Wu, Wang: "Pyrrolidine Dithiocarbamate Inhibits Nuclear Factor ?B and Toll-Like Receptor 4 Expression in Rats with Acute Necrotizing Pancreatitis." in: Gut and liver, Vol. 9, Issue 3, pp. 411-6, (2015) (PubMed).

Liu, Hu, Lian, Liu, Liu, Liu, Lin, Liu, Duan, Yong, Ye: "Dual-color immunofluorescent labeling with quantum dots of the diabetes-associated proteins aldose reductase and Toll-like receptor 4 in the kidneys of diabetic rats." in: International journal of nanomedicine, Vol. 10, pp. 3651-62, (2015) (PubMed).

Wang, Wang, Fang, Zhou, Liu, Su: "High glucose induces and activates Toll-like receptor 4 in endothelial cells of diabetic retinopathy." in: Diabetology & metabolic syndrome, Vol. 7, pp. 89, (2015) (PubMed).

Li, Hu, Yang, Zhang, Waqas, Liu, Zhang, Wang, Chen, Le, Ullah, Chen: "Expression of TLR2/4 in the sperm-storing oviduct of the Chinese soft-shelled turtle Pelodiscus sinensis during hibernation season." in: Ecology and evolution, Vol. 5, Issue 19, pp. 4466-79, (2015) (PubMed).

Li, Qian, Ju, Wang: "Upregulation of Toll-like receptor 2 expression in colorectal cancer infected by human cytomegalovirus." in: Oncology letters, Vol. 9, Issue 1, pp. 365-370, (2014) (PubMed).

Yuan, Zhang, Yang: "Ligusticum wallichii Extract Inhibited the Expression of IL-1? after AMI in Rats." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2014, pp. 620359, (2014) (PubMed).

Target Details for TLR4

Target: TLR4 (Toll-Like Receptor 4 (TLR4))

Alternative Name: TLR4 (TLR4 Products)

Synonyms: ARMD10 antibody, CD284 antibody, TLR-4 antibody, TOLL antibody, Lps antibody, Ly87 antibody, Ran/M1 antibody, Rasl2-8 antibody, toll like receptor 4 antibody, toll-like receptor 4 antibody, TLR4 antibody, Tlr4 antibody

Background: TLR4, Toll-like receptor 4, is a protein that in humans is encoded by the TLR4 gene. TLR 4 is a toll-like receptor. TLR4, the human homolog of Drosophila Toll, is a type I transmembrane protein with an extracellular domain consisting of a leucine-rich repeat region and an intracellular domain homologous to that of human interleukin-1 receptor. The TLR4 gene is mapped to chromosome 9q32-q33 by fluorescence in situ hybridization.It detects lipopolysaccharide from Gram-negative bacteria and is thus important in the activation of the innate immune system.The protein encoded by this gene is a member of the Toll-like receptor(TLR) family, which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns(PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity.

Synonyms: ARMD10 antibody|CD284 antibody|CD284 antigen antibody|Homolog of Drosophila toll antibody|hToll antibody|TLR 4 antibody|TLR4 antibody|TLR4_HUMAN antibody|Toll(Drosophila) homolog antibody|TOLL antibody|Toll Endotoxin Hyporesponsiveness antibody|Toll like receptor 4 antibody|Toll like receptor 4 precursor antibody|Toll-like receptor 4 antibody

UniProt: O00206

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades, Inflammasome, S100 Proteins