H2A Histone Family, Member V (H2AFV) (C-Term) antibody

Details for Product No. ABIN4889665, Supplier: Log in to see
Antigen
  • H2A.Z-2
  • H2afv
  • H2AV
  • h2av
  • C530002L11Rik
  • H2a.z-2
  • H2av
  • H2A.F/Z
  • h2afv
  • h2afz
  • H2A histone family, member V
  • H2A histone family member V
  • H2A histone family member V S homeolog
  • H2A histone family, member Va
  • H2afv
  • LOC100226437
  • H2AFV
  • h2afv.S
  • h2afva
Epitope
C-Term
6
3
2
1
1
1
1
Reactivity
Fruit Fly (Drosophila melanogaster)
13
7
1
1
1
1
1
1
1
1
1
Host
Rabbit
12
8
Clonality
Polyclonal
Conjugate
Un-conjugated
2
1
1
Application
ChIP DNA-Sequencing (ChIPSeq), Chromatin Immunoprecipitation (ChIP), Western Blotting (WB)
12
12
7
4
4
3
2
2
2
1
1
Options
Supplier
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Immunogen This Histone H2Av antibody was raised against a peptide in the C-terminus of the Drosophila melanogaster histone variant H2Av.
Isotype IgG
Purification Affinity Purified
Antigen
Alternative Name Histone H2Av (H2AFV Antibody Abstract)
Background The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H2A.Z (H2AZ, H2AFZ) is a histone H2A variant, a protein similar to canonical H2A but with different molecular identity and unique functions. H2A.Z is highly conserved during evolution. It plays an important role in basic cellular mechanisms such as gene activation, chromosome segregation, heterochromatic silencing and progression through the cell cycle.In Drosophila, the H2A variant corresponding to H2AZ is H2Av. H2Av is an essential protein in Drosophila and has been implicated in both activation and repression of transcription. H2Av is localized to centromeric heterochromatin in Drosophila and flies lacking H2Av have reduced levels of heterochromatin components at the centromeres. However, H2Av nucleosome distribution throughout the rest of the Drosophila genome correlates with genes that have an open and uniform chromatin architecture at promoter regions.
Molecular Weight 15 kDa
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice

Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Storage -20 °C
Storage Comment Antibodies in solution can be stored at -20 °C for 2 years.
Expiry Date 6 months
Supplier Images
ChIP DNA-Sequencing (ChIPSeq) image for anti-H2A Histone Family, Member V (H2AFV) (C-Term) antibody (ABIN4889665) Histone H2Av antibody tested by ChIP-Seq. ChIP was performed using the ChIP-IT® High ...
Western Blotting (WB) image for anti-H2A Histone Family, Member V (H2AFV) (C-Term) antibody (ABIN4889665) Histone H2Av antibody tested by Western blot. Detection of Histone H2Av by Western bl...
Product cited in: Egan, Yuan, Craske, Labhart, Guler, Arnott, Maile, Busby, Henry, Kelly, Tindell, Jhunjhunwala, Zhao, Hatton, Bryant, Classon, Trojer: "An Alternative Approach to ChIP-Seq Normalization Enables Detection of Genome-Wide Changes in Histone H3 Lysine 27 Trimethylation upon EZH2 Inhibition." in: PLoS ONE, Vol. 11, Issue 11, pp. e0166438, 2016 (PubMed).

Weber, Ramachandran, Henikoff: "Nucleosomes are context-specific, H2A.Z-modulated barriers to RNA polymerase." in: Molecular cell, Vol. 53, Issue 5, pp. 819-30, 2014 (PubMed).

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