APE antibody

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Antigen
Reactivity
Human, Mouse (Murine), Primate, Rat (Rattus)
27
11
10
9
8
Host
Mouse
17
7
4
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
2
1
1
1
1
1
1
1
1
1
1
1
Application
Chromatin Immunoprecipitation (ChIP), Gel Shift (GS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Proximity Ligation Assay (PLA), Simple Western (SimWes), Western Blotting (WB)
27
12
11
10
10
9
5
3
2
1
1
1
1
1
1
Options
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Immunogen Purified human APE1 [UniProt# P27695]
Clone 13B8E5C2
Isotype IgG2b
Purification Protein G purified
Antigen
Background Gene Symbol: APEX1
Molecular Weight Theoretical MW: 37 kDa
Gene ID 328
UniProt P27695
Application Notes Western Blot 1:100-1:2000, Simple Western 1:25, Chromatin Immunoprecipitation 1:10-1:500, Immunohistochemistry 1:100, Immunocytochemistry/Immunofluorescence 1:50-1:200, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 1:100, Immunohistochemistry-Frozen 1:10-1:500, Gel Super Shift Assays, Proximity Ligation AssayThis APE1 (13B8E5C2) antibody is useful for Western Blot, Chromatin Immunoprecipitation, Immunocytochemistry/Immunofluorescence, Immunoprecipitation and Immunohistochemistry. Gel Super Shift Assays was reported in scientific literature. In WB this antibody detects a single band at 37 kDa. In IHC, it can be competitively inhibited from recognizing the APE1 antigen in tissues using APE1 protein. It can also be used on frozen and fixed-paraffin sections and cytospin preps. In IHC-P, staining was observed in the nucleus of a human breast cancer xenograft. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer ( pH 6.0) is recommended. In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in Proximity Ligation Assay reported in scientific literature (PMID 27808278).
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer Tris-Glycine and 0.15M NaCl
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
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