RUNX3 antibody (Runt-Related Transcription Factor 3) (AA 186-415)

Details for Product anti-RUNX3 Antibody No. ABIN4899364, Supplier: Log in to see
Antigen
  • runxb
  • RUNX3
  • CBFA3
  • frrunx3
  • runx3
  • aml2
  • cbfa3
  • pebp2ac
  • AML2
  • Cbfa3
  • Pebp2a3
  • PEBP2aC
  • runt-related transcription factor 3
  • runt related transcription factor 3
  • runt related transcription factor 3 L homeolog
  • runx3
  • RUNX3
  • runx3.L
  • Runx3
Epitope
AA 186-415
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Reactivity
Human, Mouse (Murine)
132
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2
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Host
Mouse
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Clonality (Clone)
Monoclonal ()
Conjugate
This RUNX3 antibody is un-conjugated
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Application
Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Simple Western (SimWes), Western Blotting (WB)
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40
23
16
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Options
Supplier
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Supplier Product No.
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Immunogen E. coli-derived recombinant human RUNX3/CBFA3, Lys186-Tyr415, Accession # Q13761
Clone 527327
Isotype IgG2a
Specificity Detects human and mouse RUNX3/CBFA3 in Western blots.
Purification Protein A or G purified from hybridoma culture supernatant
Sterility 0.2 μm filtered
Plasmids, Primers & others Plasmids, Primers & others RUNX3 products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name RUNX3/CBFA3 (RUNX3 Antibody Abstract)
Background Alternative Names: Acute myeloid leukemia 2 protein, AML2, AML2SL3/AKV core-binding factor alpha C subunit, CBFA3, CBFA3MGC16070, CBF-alpha-3, PEA2 alpha C, PEA2-alpha C, PEBP2 alpha C, PEBP2A3, PEBP2A3FLJ34510, PEBP2AC, PEBP2-alpha C, runt domain, alpha subunit 3, runt-related transcription factor 3, SL3-3 enhancer factor 1 alpha C subunit, transcription factor AML2
Gene ID 864
UniProt Q13761
Application Notes Western Blot 1 μg/mL
Simple Western 10 μg/mL
Intracellular Staining by Flow Cytometry 2.5 μg/10^6 cells
Immunocytochemistry 8-25 μg/mL
CyTOF-ready
Restrictions For Research Use only
Reconstitution Reconstitute at 0.5 mg/mL in sterile PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C,-20 °C,-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.
Expiry Date 12 months
Supplier Images
Simple Western (SimWes) image for anti-Runt-Related Transcription Factor 3 (RUNX3) (AA 186-415) antibody (ABIN4899364) Detection of Human RUNX3/CBFA3 by Simple WesternTM. Simple Western lane view shows ly...
Flow Cytometry (FACS) image for anti-Runt-Related Transcription Factor 3 (RUNX3) (AA 186-415) antibody (ABIN4899364) Detection of RUNX3/CBFA3 in Human PBMC by Flow Cytometry. Human PBMC unstimulated (li...
Western Blotting (WB) image for anti-Runt-Related Transcription Factor 3 (RUNX3) (AA 186-415) antibody (ABIN4899364) Detection of Human and Mouse RUNX3/CBFA3 by Western Blot. Western blot shows lysates ...
Immunocytochemistry (ICC) image for anti-Runt-Related Transcription Factor 3 (RUNX3) (AA 186-415) antibody (ABIN4899364) RUNX3/CBFA3 in Human PBMCs. RUNX3/CBFA3 was detected in immersion fixed human periphe...
Product cited in: Chopin, Seillet, Chevrier, Wu, Wang, Morse, Belz, Nutt: "Langerhans cells are generated by two distinct PU.1-dependent transcriptional networks." in: The Journal of experimental medicine, Vol. 210, Issue 13, pp. 2967-80, 2013 (PubMed). Method employed by authors: Flow Cytometry (FACS), Flow Cytometry (FACS), Flow Cytometry (FACS) (Sample species: Mouse (Murine)).

Giambra, Jenkins, Wang, Lam, Shevchuk, Nemirovsky, Wai, Gusscott, Chiang, Aster, Humphries, Eaves, Weng: "NOTCH1 promotes T cell leukemia-initiating activity by RUNX-mediated regulation of PKC-? and reactive oxygen species." in: Nature medicine, Vol. 18, Issue 11, pp. 1693-8, 2012 (PubMed). Method employed by authors: Western Blotting (WB), Western Blotting (WB), Western Blotting (WB) (Sample species: Mouse (Murine)).

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