TIE1 antibody (tyrosine Kinase with Immunoglobulin-Like and EGF-Like Domains 1) (AA 22-760)

Details for Product anti-TIE1 Antibody No. ABIN4899695, Supplier: Log in to see
Antigen
  • JTK14
  • TIE
  • D430008P04Rik
  • tie-1
  • tyrosine kinase with immunoglobulin like and EGF like domains 1
  • tyrosine kinase with immunoglobulin-like and EGF-like domains 1
  • tyrosine-protein kinase receptor Tie-1
  • endothelium-specific receptor tyrosine kinase 1
  • TIE1
  • Tie1
  • CpipJ_CPIJ018933
  • tie1
Alternatives
anti-Human TIE1 antibody for Enzyme Immunoassay
Epitope
AA 22-760
15
14
12
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8
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6
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4
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3
3
2
2
2
1
1
Reactivity
Human
178
65
37
4
2
Host
Goat
119
66
2
1
Clonality
Polyclonal
Conjugate
This TIE1 antibody is un-conjugated
9
7
7
7
5
4
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
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1
1
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1
1
1
Application
Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB)
146
108
51
31
21
15
13
11
8
6
4
1
1
1
1
Options
Supplier
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Supplier Product No.
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Immunogen Mouse myeloma cell line NS0-derived recombinant human Tie-1, Ala22-Gln760, Accession # P35590
Isotype IgG
Specificity Detects human Tie-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 50 % cross-reactivity with recombinant mouse Tie-1 is observed and less than 1 % cross-reactivity with recombinant human Tie-2 is observed.
Purification Antigen Affinity-purified
Sterility 0.2 μm filtered
Plasmids, Primers & others Plasmids, Primers & others TIE1 products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name Tie-1 (TIE1 Antibody Abstract)
Background Alternative Names: EC 2.7.10, EC 2.7.10.1, JTK14soluble TIE1 variant 3, receptor tyrosine kinase, soluble TIE1 variant 1, soluble TIE1 variant 2, soluble TIE1 variant 5, Tie1, TIEsoluble TIE1 variant 4, tyrosine kinase with immunoglobulin and epidermal growth factor homologydomains 1, tyrosine kinase with immunoglobulin-like and EGF-like domains 1, tyrosine-protein kinase receptor Tie-1
Gene ID 7075
UniProt P35590
Pathways RTK Signaling
Application Notes Western Blot 0.1 μg/mL
Flow Cytometry 2.5 μg/10^6 cells
Immunohistochemistry 5-15 μg/mL
CyTOF-ready
Restrictions For Research Use only
Reconstitution Reconstitute at 0.2 mg/mL in sterile PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C,-20 °C,-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.
Expiry Date 12 months
Supplier Images
Flow Cytometry (FACS) image for anti-tyrosine Kinase with Immunoglobulin-Like and EGF-Like Domains 1 (TIE1) (AA 22-760) antibody (ABIN4899695) Detection of Tie-1 in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein...
Immunohistochemistry (IHC) image for anti-tyrosine Kinase with Immunoglobulin-Like and EGF-Like Domains 1 (TIE1) (AA 22-760) antibody (ABIN4899695) Tie-1 in Human Placenta. Tie-1 was detected in immersion fixed paraffin-embedded sect...
Product cited in: Leppänen, Saharinen, Alitalo: "Structural basis of Tie2 activation and Tie2/Tie1 heterodimerization." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 114, Issue 17, pp. 4376-4381, 2017 (PubMed). Method employed by authors: Western Blotting (WB), Western Blotting (WB) (Sample species: Human).

Morooka, Futaki, Sato-Nishiuchi, Nishino, Totani, Shimono, Nakano, Nakajima, Mochizuki, Sekiguchi: "Polydom Is an Extracellular Matrix Protein Involved in Lymphatic Vessel Remodeling." in: Circulation research, Vol. 120, Issue 8, pp. 1276-1288, 2017 (PubMed). Method employed by authors: Immunohistochemistry (IHC) (Sample species: Mouse (Murine)).

Hakanpaa, Sipila, Leppanen, Gautam, Nurmi, Jacquemet, Eklund, Ivaska, Alitalo, Saharinen: "Endothelial destabilization by angiopoietin-2 via integrin β1 activation." in: Nature communications, Vol. 6, pp. 5962, 2015 (PubMed). Method employed by authors: Immunofluorescence (IF), Immunofluorescence (IF), Immunofluorescence (IF) (Sample species: Human).

Marron, Singh, Tahir, Kavumkal, Kim, Koh, Brindle: "Regulated proteolytic processing of Tie1 modulates ligand responsiveness of the receptor-tyrosine kinase Tie2." in: The Journal of biological chemistry, Vol. 282, Issue 42, pp. 30509-17, 2007 (PubMed). Method employed by authors: Western Blotting (WB), Western Blotting (WB), Western Blotting (WB) (Sample species: Human).

Kim, Shin, Kim, Choi, Byun, Jeon, Suh, Kim: "Interaction between Tie receptors modulates angiogenic activity of angiopoietin2 in endothelial progenitor cells." in: Cardiovascular research, Vol. 72, Issue 3, pp. 394-402, 2006 (PubMed). Method employed by authors: Western Blotting (WB), Western Blotting (WB), Western Blotting (WB) (Sample species: Human).

Danet, Pan, Luongo, Bonnet, Simon: "Expansion of human SCID-repopulating cells under hypoxic conditions." in: The Journal of clinical investigation, Vol. 112, Issue 1, pp. 126-35, 2003 (PubMed). Method employed by authors: Flow Cytometry (FACS), Flow Cytometry (FACS), Flow Cytometry (FACS) (Sample species: Human).

Marron, Hughes, Edge, Forder, Brindle: "Evidence for heterotypic interaction between the receptor tyrosine kinases TIE-1 and TIE-2." in: The Journal of biological chemistry, Vol. 275, Issue 50, pp. 39741-6, 2001 (PubMed). Method employed by authors: Immunoprecipitation (IP), Immunoprecipitation (IP), Immunoprecipitation (IP) (Sample species: Human).

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