CD80 antibody (CD80) (AA 37-245)

Details for Product anti-CD80 Antibody No. ABIN4900307, Supplier: Log in to see
Antigen
  • B7
  • B7-1
  • B7.1
  • BB1
  • CD28LG
  • CD28LG1
  • LAB7
  • B71
  • Cd28l
  • Ly-53
  • Ly53
  • MIC17
  • TSA1
  • CD80 molecule
  • Cd80 molecule
  • CD80 antigen
  • CD80
  • Cd80
Alternatives
anti-Human CD80 antibody for Immunoprecipitation
Epitope
AA 37-245
33
24
19
15
15
15
14
11
10
10
8
7
6
5
5
5
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3
2
2
1
1
1
1
1
1
1
1
Reactivity
Mouse (Murine)
484
294
48
11
10
6
4
3
2
2
1
1
Host
Goat
430
125
100
94
47
16
Clonality
Polyclonal
Conjugate
This CD80 antibody is un-conjugated
76
75
56
44
21
19
19
17
10
9
9
7
7
7
7
7
7
6
6
6
6
6
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4
4
4
4
4
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Cytometry by Time of Flight (CyTOF), ELISA (Capture), Flow Cytometry (FACS), Immunocytochemistry (ICC), Neutralization (Neut), Western Blotting (WB)
554
210
170
155
119
91
81
42
33
26
24
21
12
8
7
6
5
5
5
5
4
2
2
2
2
1
1
1
Options
Supplier
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Supplier Product No.
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Immunogen Mouse myeloma cell line NS0-derived recombinant mouse B7-1/CD80, Asp37-Lys245, Accession # Q00609
Isotype IgG
Specificity Detects mouse B7-1/CD80 in ELISAs and Western blots.
Purification Antigen Affinity-purified
Sterility 0.2 μm filtered
Endotoxin Level <0.10 EU per 1 μg of the antibody by the LAL method.
Plasmids, Primers & others Plasmids, Primers & others CD80 products on genomics-online (e.g. as negative or positive controls)
Alternative Name B7-1/CD80 (CD80 Antibody Abstract)
Background Alternative Names: Activation B7-1 antigen, B7, B71, BB1, CD28LG1, CD28LGB7-1 antigen), CD80, CD80 antigen, CD80 Molecule, costimulatory factor CD80, costimulatory molecule variant IgV-CD80, CTLA-4 counter-receptor B7.1, T-lymphocyte activation antigen CD80
Gene ID 941
UniProt Q00609
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Immune Effector Process
Application Notes Western Blot 1 μg/mL
Flow Cytometry 0.25 μg/10^6 cells
Neutralization 0.15-0.6 μg/mL
Immunocytochemistry 5-15 μg/mL
CyTOF-ready
ELISA Capture (Matched Antibody Pair) 0.2-0.8 μg/mL
Restrictions For Research Use only
Reconstitution Reconstitute at 0.2 mg/mL in sterile PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C,-20 °C,-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.
Expiry Date 12 months
Supplier Images
Flow Cytometry (FACS) image for anti-CD80 (CD80) (AA 37-245) antibody (ABIN4900307) Detection of B7-1/CD80 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes eith...
Western Blotting (WB) image for anti-CD80 (CD80) (AA 37-245) antibody (ABIN4900307) Detection of Mouse B7-1/CD80 by Western Blot. Western blot shows lysates of C2C12 mou...
Neutralization (Neut) image for anti-CD80 (CD80) (AA 37-245) antibody (ABIN4900307) IL-2 secretion Induced by B7-1/CD80 and Neutralization by Mouse B7-1/CD80 Antibody. R...
Immunocytochemistry (ICC) image for anti-CD80 (CD80) (AA 37-245) antibody (ABIN4900307) anti-CD80 (CD80) (AA 37-245) antibody (Image 4)
Product cited in: Garg, Delaney, Toubai, Ghosh, Reddy, Banerjee, Yung: "Aging is associated with increased regulatory T-cell function." in: Aging cell, Vol. 13, Issue 3, pp. 441-8, 2015 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)).

Sofra, Mansour, Liu, Gao, Primpidou, Wang, Li: "Antigen-loaded ER microsomes from APC induce potent immune responses against viral infection." in: European journal of immunology, Vol. 39, Issue 1, pp. 85-95, 2009 (PubMed). Method employed by authors: Western Blotting (WB), Western Blotting (WB) (Sample species: Mouse (Murine)).

He, Li, Chen, Zhang, Chen, Hayashida, Zhu, Tseng: "Suppression of activation and induction of apoptosis in RAW264.7 cells by amniotic membrane extract." in: Investigative ophthalmology & visual science, Vol. 49, Issue 10, pp. 4468-75, 2008 (PubMed). Method employed by authors: Immunocytochemistry (ICC), Immunocytochemistry (ICC) (Sample species: Mouse (Murine)).