AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (pSer473)

Details for Product anti-AKT1 Antibody No. ABIN4900619
Antigen
  • AKT
  • CWS6
  • PKB
  • PKB-ALPHA
  • PRKBA
  • RAC
  • RAC-ALPHA
  • Akt
  • Rac
  • PKB/Akt
  • PKBalpha
  • AKT/PKB
  • AKT1
  • Akt/PKB
  • Akt1
  • CG4006
  • D-Akt
  • DAKT1
  • DAKT1/PKB
  • DAkt
  • DAkt1
  • DPKB
  • DRAC-PK
  • DRAC-PK66
  • DRAC-PK85
  • Dakt
  • Dakt1
  • Dmel\\CG4006
  • Dpkb
  • PKB/AKT
  • PKB/dAKT
  • RacPK
  • akt
  • akt1
  • dAKT
  • dAKT/dPKB
  • dAKT1
  • dAkt
  • dAkt/PKB
  • dAkt1
  • dPKB
  • dakt
  • dakt1
  • l(3)04226
  • l(3)89Bq
  • p-Akt
  • pAkt
  • ACT-5
  • akt-1
  • pkb
  • v-akt
  • v-akt1
  • xAct
  • ATAKT1
  • F18A8.2
  • F18A8_2
  • K+ transporter 1
  • POTASSIUM TRANSPORTER
  • AKT serine/threonine kinase 1
  • thymoma viral proto-oncogene 1
  • CG4006 gene product from transcript CG4006-RE
  • actin beta
  • v-akt murine thymoma viral oncogene homolog 1 S homeolog
  • v-akt murine thymoma viral oncogene homolog 1
  • K+ transporter 1
  • Serine/threonine-protein kinase akt-1
  • AKT1
  • Akt1
  • ACTB
  • akt1.S
  • akt1
  • KT1
  • akt-1
Alternatives
anti-Rat (Rattus) AKT antibody for Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections)
Epitope
pSer473
181
92
85
72
50
50
44
30
29
25
24
24
19
18
17
17
15
14
13
13
13
11
11
10
10
10
10
10
9
8
7
7
7
6
6
5
5
5
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
1321
742
619
56
53
53
34
33
30
28
6
6
5
4
4
3
3
2
1
1
1
1
Host
Rabbit
1096
257
11
4
2
1
Clonality
Polyclonal
Conjugate
This AKT antibody is un-conjugated
63
37
33
32
29
26
19
19
19
19
18
18
4
4
4
4
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
Application
Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunohistochemistry (IHC), Simple Western (SimWes), Western Blotting (WB)
1101
612
596
225
162
135
135
59
54
34
32
18
13
11
8
8
5
3
3
2
1
1
1
1
1
Options
Immunogen Phosphopeptide containing human, mouse, and rat Akt1 S473 site
Isotype IgG
Specificity Detects human, mouse and rat Akt1, Akt2 and Akt3, when phosphorylated at S473, S474 and S472, respectively.
Purification Antigen and protein A Affinity-purified
Sterility 0.2 μm filtered
Plasmids, Primers & others Plasmids, Primers & others AKT products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name Akt (AKT1 Antibody Abstract)
Gene ID 207
Pathways PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling
Application Notes Western Blot 0.5 μg/mL
Simple Western 5 μg/mL
Immunohistochemistry 5-15 μg/mL
Intracellular Staining by Flow Cytometry 2.5 μg/10^6 cells
CyTOF-ready
Restrictions For Research Use only
Reconstitution Reconstitute at 0.2 mg/mL in sterile PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C,-20 °C,-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.
Expiry Date 12 months
Supplier Images
Simple Western (SimWes) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer473) antibody (ABIN4900619) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer473) antibody
Immunohistochemistry (IHC) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer473) antibody (ABIN4900619) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer473) antibody (Image 2)
Flow Cytometry (FACS) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer473) antibody (ABIN4900619) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer473) antibody (Image 3)
Western Blotting (WB) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer473) antibody (ABIN4900619) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer473) antibody (Image 4)
Product cited in: Huang, Salavaggione, Rivera, Mukherjee, Brekken, Tennant, Iyer, Adjei: "Woodchuck VEGF (wVEGF) characteristics: Model for angiogenesis and human hepatocellular carcinoma directed therapies." in: Archives of biochemistry and biophysics, Vol. 661, pp. 97-106, 2019 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Primate).

Agod, Pazmandi, Bencze, Vereb, Biro, Szabo, Rajnavolgyi, Bacsi, Engel, Lanyi: "Signaling Lymphocyte Activation Molecule Family 5 Enhances Autophagy and Fine-Tunes Cytokine Response in Monocyte-Derived Dendritic Cells via Stabilization of Interferon Regulatory Factor 8." in: Frontiers in immunology, Vol. 9, pp. 62, 2019 (PubMed). Method employed by authors: Western Blotting (WB), Western Blotting (WB) (Sample species: Human).

Belloni, Heltai, Ponzoni, Villa, Vergani, Pecciarini, Marcatti, Girlanda, Tonon, Ciceri, Caligaris-Cappio, Ferrarini, Ferrero: "Modeling multiple myeloma-bone marrow interactions and response to drugs in a 3D surrogate microenvironment." in: Haematologica, Vol. 103, Issue 4, pp. 707-716, 2018 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Human).

Xiao, Egger, McMasters, Hao: "Differential expression of ABCB5 in BRAF inhibitor-resistant melanoma cell lines." in: BMC cancer, Vol. 18, Issue 1, pp. 675, 2018 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Human).

Kim, Jeong, Lee, Kim, Choe, Kwon, Cho, Lee: "Phosphoinositide 3-kinase-delta could be a biomarker for eosinophilic nasal polyps." in: Scientific reports, Vol. 8, Issue 1, pp. 15990, 2018 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Human).

Werling, Reglodi, Banks, Salameh, Kovacs, Kvarik, Vaczy, Kovacs, Mayer, Danyadi, Lokos, Tamas, Toth, Biro, Atlasz: "Ocular Delivery of PACAP1-27 Protects the Retina From Ischemic Damage in Rodents." in: Investigative ophthalmology & visual science, Vol. 57, Issue 15, pp. 6683-6691, 2017 (PubMed). Method employed by authors: Western Blotting (WB), Western Blotting (WB) (Sample species: Mouse (Murine)).

Budai, Tőzsér, Benkő: "Different dynamics of NLRP3 inflammasome-mediated IL‑1β production in GM-CSF- and M-CSF-differentiated human macrophages." in: Journal of leukocyte biology, Vol. 101, Issue 6, pp. 1335-1347, 2017 (PubMed). Method employed by authors: Western Blotting (WB), Western Blotting (WB) (Sample species: Human).

Fischer, Kemp, Cole-Strauss, Polinski, Paumier, Lipton, Steece-Collier, Collier, Buhlinger, Sortwell: "Subthalamic Nucleus Deep Brain Stimulation Employs trkB Signaling for Neuroprotection and Functional Restoration." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 37, Issue 28, pp. 6786-6796, 2017 (PubMed). Method employed by authors: Immunohistochemistry (Frozen Sections) (IHC (fro)) (Sample species: Mouse (Murine)).

Divakar, Ramana Reddy, Cosenza, Baker, Perumal, Antonelli, Brody, Akula, Parekh, Reddy: "Dual inhibition of CDK4/Rb and PI3K/AKT/mTOR pathways by ON123300 induces synthetic lethality in mantle cell lymphomas." in: Leukemia, Vol. 30, Issue 1, pp. 86-93, 2016 (PubMed). Method employed by authors: Western Blotting (WB), Western Blotting (WB), Western Blotting (WB) (Sample species: Human).

Dicay, Hirota, Ronaghan, Peplowski, Zaheer, Carati, MacNaughton: "Interferon-γ suppresses intestinal epithelial aquaporin-1 expression via Janus kinase and STAT3 activation." in: PLoS ONE, Vol. 10, Issue 3, pp. e0118713, 2015 (PubMed). Method employed by authors: Western Blotting (WB), Western Blotting (WB), Western Blotting (WB) (Sample species: Mouse (Murine)).

Marti, Koschwanez, Pesch, Blenn, Borel: "Water-filtered infrared a irradiation in combination with visible light inhibits acute chlamydial infection." in: PLoS ONE, Vol. 9, Issue 7, pp. e102239, 2015 (PubMed). Method employed by authors: Western Blotting (WB), Western Blotting (WB) (Sample species: Human).

von Roemeling, Radisky, Marlow, Cooper, Grebe, Anastasiadis, Tun, Copland: "Neuronal pentraxin 2 supports clear cell renal cell carcinoma by activating the AMPA-selective glutamate receptor-4." in: Cancer research, Vol. 74, Issue 17, pp. 4796-810, 2014 (PubMed). Method employed by authors: Western Blotting (WB), Western Blotting (WB) (Sample species: Human).

Mao, Xu, Li, Zhang, Yin, Liu: "The role of nuclear β-catenin accumulation in the Twist2-induced ovarian cancer EMT." in: PLoS ONE, Vol. 8, Issue 11, pp. e78200, 2013 (PubMed). Method employed by authors: Western Blotting (WB), Western Blotting (WB), Western Blotting (WB) (Sample species: Human).

He, Kang, Wu, Zhang, Wang, Gao, Zhang, Huang, Liu, Kou, Zhang, Gao: "An elaborate regulation of Mammalian target of rapamycin activity is required for somatic cell reprogramming induced by defined transcription factors." in: Stem cells and development, Vol. 21, Issue 14, pp. 2630-41, 2012 (PubMed). Method employed by authors: Western Blotting (WB), Western Blotting (WB), Western Blotting (WB) (Sample species: Mouse (Murine)).

Bifulco, Longanesi-Cattani, Franco, Pavone, Mugione, Di Carluccio, Masucci, Arra, Pirozzi, Stoppelli, Carriero: "Single amino acid substitutions in the chemotactic sequence of urokinase receptor modulate cell migration and invasion." in: PLoS ONE, Vol. 7, Issue 9, pp. e44806, 2012 (PubMed). Method employed by authors: Western Blotting (WB), Western Blotting (WB), Western Blotting (WB) (Sample species: Human).

Maddigan, Truitt, Arsenault, Freywald, Allonby, Dean, Narendran, Xiang, Weng, Napper, Freywald: "EphB receptors trigger Akt activation and suppress Fas receptor-induced apoptosis in malignant T lymphocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 187, Issue 11, pp. 5983-94, 2011 (PubMed). Method employed by authors: Western Blotting (WB), Western Blotting (WB), Western Blotting (WB) (Sample species: Human).

Deng, Han, Yan, Tian, Tao, Kang, Li: "Overexpressing cellular repressor of E1A-stimulated genes protects mesenchymal stem cells against hypoxia- and serum deprivation-induced apoptosis by activation of PI3K/Akt." in: Apoptosis : an international journal on programmed cell death, Vol. 15, Issue 4, pp. 463-73, 2010 (PubMed). Method employed by authors: Western Blotting (WB), Western Blotting (WB), Western Blotting (WB) (Sample species: Rat (Rattus)).

Han, Wu, Deng, Tao, Guo, Tian, Kang, Zhang, Yan: "Cellular repressor of E1A-stimulated genes inhibits human vascular smooth muscle cell apoptosis via blocking P38/JNK MAP kinase activation." in: Journal of molecular and cellular cardiology, Vol. 48, Issue 6, pp. 1225-35, 2010 (PubMed). Method employed by authors: Western Blotting (WB), Western Blotting (WB), Western Blotting (WB) (Sample species: Human).

Wang, Haile, Comuzzi, Tien, Wang, Yong, Jelescu-Bodos, Blaszczyk, Vessella, Masri, Sadar: "Osteoblast-derived factors induce an expression signature that identifies prostate cancer metastasis and hormonal progression." in: Cancer research, Vol. 69, Issue 8, pp. 3433-42, 2009 (PubMed). Method employed by authors: Western Blotting (WB), Western Blotting (WB), Western Blotting (WB) (Sample species: Human).

Han, Cui, Tao, Guo, Guo, Sun, Kang, Zhang, Yan, Li: "CREG inhibits migration of human vascular smooth muscle cells by mediating IGF-II endocytosis." in: Experimental cell research, Vol. 315, Issue 19, pp. 3301-11, 2009 (PubMed). Method employed by authors: Western Blotting (WB), Western Blotting (WB), Western Blotting (WB) (Sample species: Human).

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