MARCKS antibody

Catalog No. ABIN4904308

Product Details anti-MARCKS Antibody

Target: MARCKS (Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MARCKS antibody is un-conjugated

Application: Western Blotting (WB)

Characteristics: The expression of a major cellular substrate for protein kinase C, the MARCKS protein, is regulated in a cell-, tissue-, and developmental stage-specific fashion, in addition, this expression can be stimulated acutely by various cytokines in certain cell types (1). MARCKS) binds calmodulin and is a predominant, specific substrate of PKC which is phosphorylated during macrophage and neutrophil activation, growth factor-dependent mitogenesis and neurosecretion, it is redistributed from plasma membrane to cytoplasm when phosphorylated and is involved in leukocyte motility. It has been reported that MARCKS is a filamentous (F) actin crosslinking protein, with activity that is inhibited by PKC-mediated phosphorylation and by binding to calcium-calmodulin. MARCKS may be a regulated crossbridge between actin and the plasma membrane, and modulation of the actin crosslinking activity of the MARCKS protein by calmodulin and phosphorylation represents a potential convergence of the calcium-calmodulin and PKC signal transduction pathways in the regulation of the actin cytoskeleton (2).

Purification: Affinity purification

Immunogen: A synthetic peptide of human MARCKS.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Store at -20°C.

Target Details for MARCKS

Target: MARCKS (Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS))

Alternative Name: MARCKS (MARCKS Products)

Synonyms: 80K-L antibody, MACS antibody, PKCSL antibody, PRKCSL antibody, KINC antibody, Macs antibody, marcks antibody, MGC89127 antibody, MARCKS antibody, macs antibody, wu:fb55c06 antibody, wu:fb92d04 antibody, wu:fc19b04 antibody, wu:fc44a10 antibody, zgc:110235 antibody, fc34g03 antibody, fc38c06 antibody, wu:fc34g03 antibody, wu:fc38c06 antibody, zgc:109978 antibody, myristoylated alanine rich protein kinase C substrate antibody, myristoylated alanine-rich protein kinase C substrate antibody, methyl binding domain106 antibody, myristoylated alanine-rich protein kinase C substrate b antibody, myristoylated alanine-rich protein kinase C substrate S homeolog antibody, myristoylated alanine-rich protein kinase C substrate a antibody, MARCKS antibody, Marcks antibody, marcks antibody, mbd106 antibody, marcksb antibody, marcks.S antibody, marcksa antibody

Gene ID: 4082

UniProt: P29966