HUWE1 antibody (AA 4251-4263)

Catalog No. ABIN490548

Product Details anti-HUWE1 Antibody

Target: HUWE1 (HECT, UBA and WWE Domain Containing 1, E3 Ubiquitin Protein Ligase (HUWE1))

Binding Specificity: AA 4251-4263

Reactivity: Human, Mouse, Rat, Dog, Hamster, Horse, Monkey, Pig, Rabbit, Bat, Xenopus laevis, Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This HUWE1 antibody is un-conjugated

Application: ELISA

Specificity: Human HUWE1.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit, Pig, Opossum, Platypus, Lizard, Xenopus (100%) Salmon, Stickleback, Pufferfish, Zebrafish, Beetle (92%) Seq squirt, Water flea (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-DDLKSNTEYHKYQ from an internal region of human HUWE1 (NP_113584.3). Percent identity by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit, Pig, Opossum, Platypus, Lizard, Xenopus (100%), Salmon, Stickleback, Pufferfish, Zebrafish, Beetle (92%), Seq squirt, Water flea (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: Peptide-ELISA (1:128000)

Usage: Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Not yet tested - our routinely used western blotting protocol does not allow detection of proteins as large as the calculated size of 482  kDa according to NP_113584.3. Therefore we cannot recommend an optimal concentration and the antibody is an aspiring p

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for HUWE1

Target: HUWE1 (HECT, UBA and WWE Domain Containing 1, E3 Ubiquitin Protein Ligase (HUWE1))

Alternative Name: HUWE1 / ARFBP1 (HUWE1 Products)

Synonyms: ARF-BP1 antibody, HECTH9 antibody, HSPC272 antibody, Ib772 antibody, LASU1 antibody, MULE antibody, URE-B1 antibody, UREB1 antibody, 5430439H10Rik antibody, AU041296 antibody, Arf-bp1 antibody, C430014N20Rik antibody, C80292 antibody, Gm1718 antibody, Mule antibody, Ureb1 antibody, HUWE1 antibody, si:dkey-242a8.2 antibody, Huwe1 antibody, mule antibody, ib772 antibody, lasu1 antibody, ureb1 antibody, hecth9 antibody, arf-bp1 antibody, hspc272 antibody, HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase antibody, HECT, UBA and WWE domain containing 1 antibody, E3 ubiquitin-protein ligase HUWE1 antibody, HUWE1 antibody, Huwe1 antibody, huwe1 antibody, LOC100123214 antibody, LOC100380641 antibody

Background: Name/Gene ID: HUWE1

Synonyms: HUWE1, ARF-binding protein 1, ARF-BP1, BJ-HCC-24 tumor antigen, Ib772, KIAA0312, LASU1, Mcl-1 ubiquitin ligase E3, HECTH9, MULE, HSPC272, KIAA1578, URE-B1, UREB1, URE-binding protein 1, HECT domain protein LASU1, Large structure of UREB1

Gene ID: 10075

NCBI Accession: NP_113584

UniProt: Q7Z6Z7