GFP Tag antibody
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- Target See all GFP Tag products
- GFP Tag
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Reactivity
- Aequorea victoria
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This GFP Tag antibody is un-conjugated
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Application
- Western Blotting (WB)
- Purification
- Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified by protein G and Ni-NTA nickel affinity chromatography, followed by dialysis against PBS.
- Immunogen
- Purified His-tagged GFP protein was used to produced this monoclonal antibody.
- Clone
- 168AT1211
- Isotype
- IgG1
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anti-GFP Tag antibody
Reactivity: Human IF, WB Host: Mouse Monoclonal 168AT1211 unconjugated
anti-GFP Tag antibodyWB Host: Mouse Monoclonal 168AT1211-269-64 unconjugated
anti-GFP Tag antibodyReactivity: Aequorea victoria IF, IHC (p), WB Host: Mouse Monoclonal 168AT1211 unconjugated
anti-GFP Tag antibodyWB Host: Rabbit Polyclonal unconjugated
anti-GFP Tag (N-Term) antibodyReactivity: Aequorea victoria IHC, ELISA, WB Host: Mouse Monoclonal LGB-1 unconjugated
anti-GFP Tag antibodyReactivity: Aequorea victoria DB, ELISA, IHC, IP, WB Host: Mouse Monoclonal 946as unconjugated
anti-GFP Tag antibodyReactivity: Aequorea victoria ELISA, ICC, IF Host: Mouse Monoclonal 15 unconjugated
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- Application Notes
- WB 1:1000-2000
- Restrictions
- For Research Use only
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- Storage
- 4 °C,-20 °C
- Storage Comment
- Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
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- Target
- GFP Tag
- Abstract
- GFP Tag Products
- Synonyms
- green fluorescent protein antibody, gfp antibody
- Target Type
- Tag
- Background
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Green fluorescent protein (GFP), originally isolated from the jellyfish Aequorea victoria, is one of the best visual reporters for monitoring gene expression in vivo and in situ. GFP is a also convenient marker for use in flow cytometry because it eliminates the need to incubate with a secondary reagent (such as dyes or antibodies) for detection. However, anti-GFP antibody is also widely used for co-immunipreciapitation, co-localization or western blotting for the confirmation of specificity when a GFP fusion protein is expressed in cells. Abgent's anti-GFP monoclonal antibody provides a simple solution to detect the expression of a GFP-tagged protein in cells. Because of its ability to spontaneously generate its own fluorophore, the green fluorescent protein (GFP) from the jellyfish Aequorea victoria is used extensively as a fluorescent marker in molecular and cell biology. The yellow fluorescent proteins (YFPs) have the longest wavelength emissions of all GFP variants examined to date. This shift in the spectrum is the result of a T203Y substitution (single-letter amino acid code), a mutation rationally designed on the basis of the X-ray structure of GFP S65T. Abgent's anti-GFP monoclonal antibody can detect both GFP and YFP but not BFP (Blue fluorescent protein) by western blotting.
