Recombinant Rabbit anti-Human IgG4 Antibody

Catalog No. ABIN4953372

Product Details

Target: IgG4

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Characteristics: This recombinant Human IgG4 antibody reacts to the heavy chain of hIgG4. No cross reactivity with human IgG1, IgG2, IgG3, IgM, IgA, IgD, IgE, mouse/rat/goat IgG.

Purification: Protein A purified from animal origin-free supernatant

Immunogen: Peptide corresponding to the hinge region of human IgG4 was used as the immunogen for this recombinant Human IgG4 antibody.

Clone: RM120

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the recombinant Human IgG4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH 6 Citrate buffer or pH 9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.\. ELISA: 50 ng/well - 200 ng/well (Capture), 0.05-0.2 μg/mL (Detection),Western Blot: 0.5-2 μg/mL,Immunocytochemistry: 1-10 μg/mL,Immunohistochemistry: 1-10 μg/mL (1)

Restrictions: For Research Use only

Handling

Buffer: 1 mg/mL in PBS with 50 % glycerol, 1 % BSA and 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the recombinant Human IgG4 antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol).

Target Details

Target: IgG4

Abstract: IgG4 Products

Target Type: Antibody

Background: This recombinant Human IgG4 antibody reacts to the heavy chain of hIgG4. No cross reactivity with human IgG1, IgG2, IgG3, IgM, IgA, IgD, IgE, mouse/rat/goat IgG.

Gene ID: 3503

UniProt: P01861