HLA-B antibody (AA 62-90)

Catalog No. ABIN3031679

Product Details anti-HLA-B Antibody

Target: HLA-B (Major Histocompatibility Complex, Class I, B (HLA-B))

Binding Specificity: AA 62-90

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HLA-B antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purification: Purified

Immunogen: A portion of amino acids 62-90 from the human protein was used as the immunogen for this HLA-B antibody.

Clone: 528CT10-4-1

Isotype: IgM

Application Details

Application Notes: Titration of the HLA-B antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:100-1:250

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the HLA-B antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for HLA-B

Target: HLA-B (Major Histocompatibility Complex, Class I, B (HLA-B))

Alternative Name: HLA-B (HLA-B Products)

Synonyms: AS antibody, HLAB antibody, SPDA1 antibody, mhc1b antibody, major histocompatibility complex, class I, B antibody, major histocompatibility complex nonclassical class Ib L homeolog antibody, HLA-B antibody, BOLA-B antibody, MAMU-B18 antibody, DKFZP469N1225 antibody, mhc1b.L antibody

Background: HLA-B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exon 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-B alleles have been described.

UniProt: P03989

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints