MYBL2 antibody

Catalog No. ABIN498105

Product Details anti-MYBL2 Antibody

Target: MYBL2 (V-Myb Myeloblastosis Viral Oncogene Homolog (Avian)-Like 2 (MYBL2))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MYBL2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Specificity: This antibody antibody detects endogenous levels of B-Myb / MYB2 protein.

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse, Rat.

Purification: Affinity Chromatography using epitope-specific immunogen

Application Details

Application Notes: Western Blots: 1/500approx. 1/1000. Immunofluorescence: 1/50approx. 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for MYBL2

Target: MYBL2 (V-Myb Myeloblastosis Viral Oncogene Homolog (Avian)-Like 2 (MYBL2))

Alternative Name: MYBL2 (MYBL2 Products)

Synonyms: MYBL2 antibody, bmyb antibody, b-myb antibody, B-Myb antibody, Bmyb antibody, B-MYB antibody, BMYB antibody, myb1 antibody, MYB proto-oncogene like 2 antibody, v-myb avian myeloblastosis viral oncogene homolog-like 2b antibody, myeloblastosis oncogene-like 2 antibody, MYB proto-oncogene like 2 S homeolog antibody, MYBL2 antibody, mybl2 antibody, mybl2b antibody, Mybl2 antibody, mybl2.S antibody

Background: Expression of the B-Myb transcription factor is upregulated during late G1 phase of the cell cycle by an E2F-dependent transcriptional mechanism. B-Myb is specifically phosphorylated during S phase, suggesting that a cyclin-dependent kinase (Cdk) regulates its activity. Consistent with this notion, the S phase-specific cyclin A/Cdk2 was found previously to enhance B-Myb transactivation activity in cotransfected cells. There is evidence that B-Myb is a direct physiological target for cyclin A/Cdk2. Data indicate that phosphorylation by cyclin A/Cdk2 is directly involved in enhancing B-Myb transactivation activity and that levels of endogenous cyclin A/Cdk2 activity may contribute to cell line-specific B-Myb function . Nuclear entry of B-Myb is dependent on multiple nuclear localization signals (NLS's). Mutagenesis of the putative NLS's of B-Myb has identified two separate NLS's, NLS1 and NLS2. Each of the two NLS's is essential for efficient nuclear targeting.Synonyms: B-Myb, BMYB, Myb-like protein 2, Myb-related protein B

Molecular Weight: approx. 80 kDa

Gene ID: 4605

NCBI Accession: NP_002457

UniProt: P10244

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, Chromatin Binding