DUSP1 antibody

Catalog No. ABIN498155

Product Details anti-DUSP1 Antibody

Target: DUSP1 (Dual Specificity Phosphatase 1 (DUSP1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DUSP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of MKP1 protein.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity chromatography using epitope-specific immunogen

Purity: > 95 % (by SDS-PAGE)

Application Details

Application Notes: Western blot: 1: 500 - 1: 1000. Immunohistochemistry on paraffin sections: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for DUSP1

Target: DUSP1 (Dual Specificity Phosphatase 1 (DUSP1))

Alternative Name: DUSP1 / MKP1 (DUSP1 Products)

Synonyms: CL100 antibody, HVH1 antibody, MKP-1 antibody, MKP1 antibody, PTPN10 antibody, 3CH134 antibody, Mkp1 antibody, Ptpn16 antibody, DUSP1 antibody, U19515 antibody, erp antibody, mkp-1 antibody, cb269 antibody, sb:cb269 antibody, zgc:55920 antibody, id:ibd2586 antibody, wu:fd19g02 antibody, wu:fe50g09 antibody, XCL100 antibody, dual specificity phosphatase 1 antibody, dual specificity phosphatase 1 S homeolog antibody, DUSP1 antibody, Dusp1 antibody, dusp1 antibody, dusp1.S antibody

Background: The expression of DUSP1 gene is induced in human skin fibroblasts by oxidative/heat stress and growth factors. It specifies a protein with structural features similar to members of the non-receptor-type protein-tyrosine phosphatase family, and which has significant amino-acid sequence similarity to a Tyr/Ser-protein phosphatase encoded by the late gene H1 of vaccinia virus. The bacterially expressed and purified DUSP1 protein has intrinsic phosphatase activity, and specifically inactivates mitogen-activated protein (MAP) kinase in vitro by the concomitant dephosphorylation of both its phosphothreonine and phosphotyrosine residues. Furthermore, it suppresses the activation of MAP kinase by oncogenic ras in extracts of Xenopus oocytes. Thus, DUSP1 may play an important role in the human cellular response to environmental stress as well as in the negative regulation of cellular proliferation.Synonyms: CL100, Dual specificity protein phosphatase 1, Dual specificity protein phosphatase hVH1, MAP kinase phosphatase 1, MKP-1, PTPN10, Protein-tyrosine phosphatase CL100, VH1

Molecular Weight: approx. 39 kDa

Gene ID: 1843

NCBI Accession: NP_004408

UniProt: P28562